Učinak bakra na genotoksičnost kadmija u duhanu, Nicotiana tabacum L.

master's thesis

• Title (english): Effect of copper on the genotoxicity of cadmium in tobacco, Nicotiana tabacum L.
• Author: Maja Ćetković
• Mentor: Mirjana Pavlica (mentor)
• Committee member: Mirjana Pavlica (predsjednik povjerenstva)
• Committee member: Zora Popović (član povjerenstva)
• Committee member: Zdravko Dolenec (član povjerenstva)
• Committee member: Vesna Petrović Peroković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2011-06-27, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Nagli razvoj industrije uzrokuje sve veće zagađenje okoliša. Među brojne okolišne zagađivače ubrajaju se i teški metali. Toksičnost teških metala ovisi o njihovoj koncentraciji, pokretljivosti u biljci i okolišnim uvjetima. Esencijalni metali kao što je bakar, dostupni u prevelikoj koncentraciji postaju toksični za biljku. Kadmij je jedan od neesencijalnih toksičnih teških metala koji je genotoksičan i mutagen za većinu organizama. Cilj istraživanja bio je utvrditi osjetljivost genoma duhana na teške metale bakar i kadmij, pojedinačno i u kombinaciji, metodom komet-testa te istražiti ima li bakar sinergističko ili antagonističko djelovanje na genotoksičnost kadmija. Bioakumulacija istraživanih metala istražena je analizom sadržaja metala u biljnom tkivu tehnikom optičke emisijske spektroskopije uz induktivno spregnutu plazmu. Analiza sadržaja metala pokazala je da je akumulacija kadmija bila viša u korijenu nego u listu biljke duhana. Komet-testom potvrđena je osjetljivost genoma duhana na kadmij i bakar. Celularnim komet-testom uočeno je antagonističko djelovanje bakra na genotoksičnost kadmija u listu, a sinergističko u korijenu. Acelularni komet-test pokazao je da bakar smanjuje genotoksični učinak kadmija u listu.

Abstract (english)

Rapid industry development causes increase of environmental pollution. Among numerous environmental contaminants, heavy metals are of significant value. Toxicity of heavy metals depends on their concentration, mobility in the plant and environmental conditions. Essential metals such as copper, when present in excessive concentration become toxic to plants. Cadmium is one of the nonessential toxic heavy metals that is genotoxic and mutagenic to organisms. The main goal of this study was to determine the sensitivity of the tobacco genome on the heavy metals copper and cadmium, individually and in mixture by comet assay and to investigate whether copper has antagonistic or synergistic effect on the cadmium genotoxicity. Bioaccumulation of heavy metals was measured with inductively coupled plasma atomic emission spectroscopy. Analysis of metal content showed that cadmium accumulation was higher in roots than in leaves of tobacco plants. Comet assay confirmed the sensitivity of the tobacco genome to cadmium and copper. Cellular comet assay showed antagonistic effect of copper on cadmium genotoxicity in leaves, and synergistic effect in roots. Acellular comet test showed that copper reduces genotoxic effects of cadmium in leaves.

• Keywords: Nicotiana tabacum L.; bakar; kadmij; genotoksičnost; komet-test; bioakumulacija
• Keywords (english): Nicotiana tabacum L.; copper; zinc; cadmium; genotoxicity; comet assay; bioacumulation
• Language: croatian
• URN:NBN: urn:nbn:hr:217:329095
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-03 07:49:23