Usmjerena evolucija kao metoda u dizajnu novih katalitičkih aktivnosti enzima

undergraduate thesis

• Title (english): Directed evolution as a method in the design of new catalytic activities of enzymes
• Author: Ivan Zubčić
• Mentor: Ita Gruić-Sovulj (mentor)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2022-09-22, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Usmjerena evolucija je relativno nova metoda u dizajniranju već postojećih proteina. Metoda se
temelji na uvođenju promjena u slijedu nukleotida DNA koja kodira za željeni protein. Nakon
uvođenja promjena u željeni slijed nukleotida, pokušava se nekom metodom selekcije ili probira
identificirati protein s promjenjenim svojstvima koja mogu sezati od od afiniteta eznima prema
supstratu do termostabilnosti samog proteina. Važno je konstruirati pogodan sustav za probir ili
selekciju koji omogćuje pronalaženje modificiranog proteina. Usmjerena evolucija je omogućila
modifikaciju proteina u vidu kataliziranja novih reakcija, kataliziranju nastanka novih kemijskih
veza, sintezi određenih molekula poput biogoriva i mnogih drugih mogućnosti.

Abstract (english)

Directed evolution is a relatively new method in designing already existing proteins. The method
is based on the introduction of a change in the DNA sequence that codes for the desired protein.
After the DNA sequence has been altered, an attempt is made by some method of selection to try
to identify a protein with changed properties ranging from the affinity of enzymes to the substrate
to the thermostability of the protein itself . It is important to construct a suitable screening/selection
system that allows finding the modified protein. Directed evolution has made it possible to modify
proteins in a way of catalyzing new reactions, catalyzing the formation of chemical bonds,
synthesizing certain molecules such as biofuels and many other possibilities.

• Keywords: usmjerena evolucija; biblioteka mutanti; ekspresijski sustavi; citokromi; biogoriva
• Keywords (english): directed evolution; library mutant; expression systems; cytochromes; biofules
• Language: croatian
• URN:NBN: urn:nbn:hr:217:279245
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-10-13 10:41:54