Utjecaj akutne neonatalne hipoksije na ekspresiju gena za vazoaktivni intestinalni peptid u hipokampusu adolescentnih štakora

master's thesis

• Title (english): The effect of acute neonatal hypoxia on the expression of vasoactive intestinal polypeptide gene in adolescent rat hippocampus
• Author: Valentina Runko
• Mentor: Dubravka Hranilović (mentor)
• Committee member: Dubravka Hranilović (predsjednik povjerenstva)
• Committee member: Silvija Černi (član povjerenstva)
• Committee member: Duje Lisičić (član povjerenstva)
• Committee member: Dubravko Pavoković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; Zagreb
• Defense date and country: 2022-04-28, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Vazoaktivni intestinalni peptid (VIP) je regulatorna molekula, koja u sisavaca ima ulogu
neurotransmitera, hormona te faktora rasta i razvoja. Unutar hipokampusa, VIP sudjeluje u kontroli
sinaptičke plastičnosti, poput dugotrajne potencijacije i dugotrajne depresije. Smatra se da neuronski
krugovi koji eksprimiraju VIP moderiraju prostorno učenje potaknuto nagradom. Ovisno o stupnju i
trajanju, prenatalna hipoksija u ljudi spada u faktore rizika za niz poremećaja, uključujući cerebralnu
paralizu, poremećaj pažnje s hiperaktivnošću, poremećaje autističnog spektra te poteškoće u prostornom
učenju i radnoj memoriji. U ovom smo radu, koristeći model akutne neonatalne hipoksije u
normobaričnim uvjetima, istražili uzrokuje li neonatalno izlaganje štakora hipoksiji promjenu u razini
ekspresije gena za VIP u hipokampusu adolescenth štakora, kod kojih je ranije opažena promjena u
prostornom učenju. Diferencijalnu ekspresiju gena za VIP istražila sam kvantitativnom lančanom
reakcijom polimerazom, korištenjem RNA prethodno izolirane iz hipokampusa 16 tretiranih i 15
kontrolnih štakora oba spola. Dvosmjerna analiza varijanci pokazala je značajan utjecaj tretmana na
ekspresiju gena za VIP, uz 2,5 puta veću ekspresiju u hipoksičnoj skupini. Zaključujemo da je akutno
neonatalno izlaganje hipoksiji moglo dovesti do dugotrajnog kompenzatornog povećanja ekspresije
gena za VIP, koje se pak može povezati s promjenama u prostornom učenju posredovanom nagradom.

Abstract (english)

Vasoactive intestinal peptide (VIP) is a regulatory molecule in the mammalian brain, acting as a
neurotransmitter, hormone, and growth factor. Within the hippocampus, VIP participates in controlling
synaptic plasticity phenomena, such as long-term potentiation and long-term depression. Neuron circuits
which express VIP are thought to moderate reward-associated learning. Depending on duration and
severity, prenatal and neonatal hypoxia is a risk factor for various disorders, including cerebral palsy,
attention-deficit hyperactive disorder, autism spectrum disorders, and difficulties in spatial learning and
working memory. In this paper, we used a model of neonatal hypoxia in normobaric conditions, in order
to explore whether neonatal exposure to hypoxia changes VIP expression in the hippocampus of
adolescent rats, which were earlier shown to have altered spatial learning. In order to explore the
differential expression of VIP gene, I used qPCR method on RNA isolated from the hippocampus of
adolescent rats of both sexes, 16 of which were subjected to hypoxia treatment, and 15 of which were
controls. Two-way ANOVA showed a significant influence of treatment on VIP gene expression, which
was 2.5 times higher in the hypoxia treated group. We conclude that acute neonatal exposure to hypoxia
may lead to a long-term compensatory increase in VIP gene expression, which can be correlated to
changes in reward-motivated spatial learning.

• Keywords: neinvazivni model; VIP; kvantitativni PCR; mRNA
• Keywords (english): non-invasive model; VIP; quantitative PCR; mRNA
• Language: croatian
• URN:NBN: urn:nbn:hr:217:992252
• Project: Number: IP-2019-04-3182; Title: Moždani izvanstanični matriks u razvitku i perinatalnoj hipoksiji; Title: Brain extracellular matrix in development and in perinatal hypoxia; Acronym: BRAINECM; Leader: Nataša Jovanov Milošević; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: IP
• Project: Number: KK.01.1.1.01.0007; Title: Eksperimentalna i klinička istraživanja hipoksijsko-ishemijskog oštećenja mozga u perinatalnoj i odrasloj dobi; Leader: Miloš Judaš; Jurisdiction: Croatia; Funder: ERDF
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2023-04-29
• Created on: 2022-10-17 08:55:16