Posljedice neonatalne hipoksije na ponašanje štakora

master's thesis

• Title (english): Consequences of neonatal hypoxia on rat behavior
• Author: Matea Krizman
• Mentor: Dubravka Hranilović (mentor)
• Committee member: Dubravka Hranilović (predsjednik povjerenstva)
• Committee member: Jasna Lajtner (član povjerenstva)
• Committee member: Božena Mitić (član povjerenstva)
• Committee member: Sofia Ana Blažević (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2019-03-08, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Prenatalna hipoksija stanje je smanjene opskrbe fetalnih tkiva kisikom koje moţe dovesti do
oštećenja organa s dugoročnim posljedicama. Kod oštećenja mozga moţe doći do mentalne
retardacije, cerebralne paralize, epilepsije i poremećaja ponašanja. Zbog toga se radi velik broj
istraţivanja u kojima vaţnu ulogu imaju animalni modeli, od kojih se najčešće koristi štakor. U
neonatalnog štakora, stadij razvoja mozga odgovara stadiju razvoja mozga fetusa čovjeka od 23. do
34. tjedna trudnoće, kad najčešće dolazi do problema s hipoksijom. Dosadašnja istraţivanja koristila
su invazivne modele hipoksije kod kojih je ponekad teško zaključiti je li neka promjena rezultat
hipoksije ili samog operacijskog zahvata. Stoga je cilj ovog istraţivanja bio razviti neinvazivni
model te utvrditi postoje li promjene u lokomociji, anksioznom, eksploratornom i socijalnom
ponašanju te učenju u mladih štakora, izloţenih hipoksiji u normobaričnoj komori prvog
postnatalnog dana. U ovom smo diplomskom radu mladunce štakora od 33. do 45. postnatalnog
dana podvrgnuli bateriji testova ponašanja: otvoreno polje, ploča s rupama, T-labirint i društveni
odabir. Razina lokomotornog gibanja, anksiozno i eksploratorno ponašanje nisu se razlikovali
izmeĎu skupina. U odnosu na kontrolnu skupinu, skupina izloţena hipoksiji imala je značajno manji
broj točnih odabira u T-labirintu, a muţjaci i produljeno vrijeme istraţivanja druge jedinke u testu
društvenog odabira. Rezultati upućuju na zaključak da neonatalno izlaganje štakora hipoksiji
smanjuje sposobnost prostornog učenja i povećava socijabilnost u muţjaka.

Abstract (english)

Prenatal hypoxia is a state of reduced oxygen supply to fetal tissue that can lead to organ damage
with long-term consequences. Brain damage can lead to mental retardation, cerebral palsy, epilepsy
and behavioral disorders. For this reason, a large amount of research has been done on animal
models, especially rats. In the neonatal rat, the stage of brain development matches the stage of
brain development in the human fetus from the 23rd to the 34th week of pregnancy, when problems
with hypoxia most often occur. Disadvantage of previously used invasive models of hypoxia is a
difficulty to conclude whether the observed changes are the result of hypoxia or the operation itself.
Therefore, the aim of this study was to develop a non-invasive model and to determine possible
changes in locomotion, anxiety-like, exploratory and social behavior, and learning in young rats
exposed to hypoxia in the normobaric chamber on the first postnatal day. In this study, young rats
underwent the battery of behavioral tests: open field, hole board, T-maze and social choice, from
the 33rd to the 34th postnatal day. The level of locomotion, anxiety-like and exploratory behavior
did not differ between the groups. Compared to the control group, the hypoxia-exposed group had a
significantly smaller number of correct choices in the T-maze. In addition males from the
experimental group displayed prolonged research time of the conspecific in the social choice test.
The findings suggest that the exposure of neonatal rats to hypoxia reduces the ability of spatial
learning and increases the sociability in males.

• Keywords: normobarična komora; neinvazivni model; socijalno ponašanje; prostorno učenje; Tlabirint
• Keywords (english): normobaric chamber; non-invasive model; social behavior; spatial learning; T-maze
• Language: croatian
• URN:NBN: urn:nbn:hr:217:728172
• Project: Number: IP-2019-04-3182; Title: Moždani izvanstanični matriks u razvitku i perinatalnoj hipoksiji; Acronym: BRAINECM; Leader: Nataša Jovanov Milošević; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: IP
• Project: Number: KK.01.1.1.01.0007; Title: Eksperimentalna i klinička istraživanja hipoksijsko-ishemijskog oštećenja mozga u perinatalnoj i odrasloj dobi; Leader: Miloš Judaš; Jurisdiction: Croatia; Funder: ERDF
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-10-17 09:20:03