Posljedice izloženosti neonatalnoj hipoksiji na ponašanje odraslih štakora

master's thesis

• Title (english): Consequences of neonatal hypoxia on behavior of adult rats
• Author: Ivona Kološnjaj
• Mentor: Dubravka Hranilović (mentor)
• Committee member: Sunčica Bosak (predsjednik povjerenstva)
• Committee member: Dubravka Hranilović (član povjerenstva)
• Committee member: Romana Gračan (član povjerenstva)
• Committee member: Sofia Ana Blažević (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2020-02-27, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Za održavanje normalne funkcije mozga ključna je dostupnost optimalne količine kisika.
Nedostatak te esencijalne molekule inducira hipoksiju. Fetalna hipoksija dovodi do smanjenja
adaptivnog potencijala mozga i plastičnosti zbog poremećaja umrežavanja neurona, posebno u
korteksu i hipokampusu. Oštećenje mozga može dovesti do ozbiljnih posljedica kao što su
mentalna retardacija, cerebralna paraliza, poremećaji pamćenja i epilepsija. Štakori, najčešći
animalni modeli hipoksije, omogućuju bolje razumijevanje manje laboratorijski pristupačnog
ljudskog mozga. Stadij razvoja mozga neonatalnog štakora odgovara stadiju razvoja mozga
fetusa čovjeka od 23. do 34. tjedna trudnoće. Cilj ovog diplomskog rada je utvrditi postoje li
promjene u socijalnom, emocionalnom i eksploratornom ponašanju te lokomociji i kogniciji u
odraslih štakora izloženih hipoksiji u hipobaričnim ili normobaričnim uvjetima prvog
postnatalnog dana, u odnosu na odrasle štakore kontrolne skupine te u kojoj mjeri one
odgovaraju promjenama uočenim u mladunaca. Razlike u lokomociji i eksploratornom
ponašanju, koje su uočene u skupini hipoksičnih mladunaca, u odnosu na kontrolnu skupinu,
nisu zadržane u odrasloj dobi. Moguće je da je jedina preostala značajna razlika u broju
propinjanja vezana uz pamćenje i procesiranje prostora posredovano dopaminom. Rezultati
upućuju na zaključak da se neke promjene u ponašanju, nastale kao posljedica izloženosti
mozga u razvoju hipoksiji, kompenziraju tijekom odrastanja, dok druge ostaju trajno prisutne.

Abstract (english)

Optimal amount of oxygen is crucial for maintaining normal brain function. The lack of this
essential molecule promotes hypoxia. Fetal hypoxia leads to a decrease in adaptive potential of
the brain and plasticity due to a disturbance in neuronal networking, especially in the cortex
and hippocampus. Brain damage can lead to serious consequences such as mental retardation,
cerebral palsy, memory disturbances and epilepsy. Rats, the most common animal models of
hypoxia, provide a better understanding of the less laboratory-accessible human brain. The
stage of brain development of neonatal rats corresponds to the stage of development of a human
fetal brain from 23rd to 34th week of pregnancy. The aim of this graduation thesis is to
determine whether there are changes in social, emotional and exploratory behaviour, as well as
locomotion and cognition in adult rats exposed to hypobaric or normobaric conditions on the
first postnatal day compared to adult rats in control group and to what extent they correspond
to changes observed in young rats. The differences in locomotion and exploratory behaviour
observed in the group of young hypoxic rats in relation to the control group were not retained
in adulthood. The only significant difference remaining in adulthood was the increased number
of rearings in the experimental groups, which may be related to memory and spatial processing
mediated by dopamine. The results suggest that some behavioural changes, caused by the
exposure of developing brain to hypoxia, compensate themseves during development, while
others remain permanently present.

• Keywords: normobarična komora; hipobarična komora; neinvazivni model; prostorno učenje; propinjanje
• Keywords (english): normobaric chamber; hypobaric chamber; non- invasive model; spatial learning; rearing
• Language: croatian
• URN:NBN: urn:nbn:hr:217:032607
• Project: Number: IP-2019-04-3182; Title: Moždani izvanstanični matriks u razvitku i perinatalnoj hipoksiji; Title: Brain extracellular matrix in development and in perinatal hypoxia; Acronym: BRAINECM; Leader: Nataša Jovanov Milošević; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: IP
• Project: Number: KK.01.1.1.01.0007; Title: Eksperimentalna i klinička istraživanja hipoksijsko-ishemijskog oštećenja mozga u perinatalnoj i odrasloj dobi; Leader: Miloš Judaš; Jurisdiction: Croatia; Funder: ERDF
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2021-02-27
• Created on: 2022-10-17 09:26:23