Dinamika specijaliziranoga metabolizma i fiziološkog odgovora vinove loze (Vitis vinifera) inficirane fitoplazmom flavescence dorée

master's thesis

• Title (english): Dynamics of specialized metabolism and physiological responses of grapevine (Vitis vinifera) infected with flavescence dorée phytoplasma
• Author: Dino Davosir
• Mentor: Martina Šeruga Musić (mentor)
• Mentor: Ivana Šola (komentor)
• Committee member: Martina Šeruga Musić (predsjednik povjerenstva)
• Committee member: Biljana Balen (član povjerenstva)
• Committee member: Ana Galov (član povjerenstva)
• Committee member: Marin Ježić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2022-09-26, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Fitoplazme (rod ‘Candidatus Phytoplasma’) su unutarstanične parazitske bakterije i patogeni
širokog kruga biljaka. Fitoplazma flavescence dorée (FDp) uzročnik je velikih šteta u
vinogradarskoj proizvodnji u Europi. U ovom istraživanju, sakupljeni su listovi simptomatskih i
asimptomatskih listova vinove loze (Vitis vinifera L.) var. 'Pinot gris' u tri vremenske točke tijekom
razvoja infekcije. FDp je detektirana korištenjem kvantitativnog PCR i genotipizirana
sekvenciranjem i filogenetskom analizom map gena, što je pokazalo prisutnost dva genotipa, M38
i M54. Korištenjem spektrofotometrijskih i kromatografskih (RP-HPLC, GC-MS) metoda,
analiziran je utjecaj dva FDp genotipa na fiziološke parametre i fenolni metabolizam vinove loze
tijekom razvoja infekcije. Genotip M54 je slabije djelovao na fiziološke parametre, ali je održavao
viši titar nego M38. Analiza korelacija pokazala je da promjene fizioloških parametara mogu biti
povezane s titrom FDp. Sadržaj svih glavnih grana fenolnog metabolizma i individualnih spojeva
je pokazao slične obrasce, ukazujući na pojačanje temeljnog fenolnog biosintetskog puta zbog
infekcije FDp, posebno u trećoj vremenskoj točki. Analizirane promjene fiziologije vinove loze
ovisne o genotipu FDp daju podatke o metama FDp u vinovoj lozi i mogu poduprijeti identifikaciju
potencijalnih efektora ove fitoplazme.

Abstract (english)

Phytoplasmas (genus ‘Candidatus Phytoplasma’) are intracellular parasitic bacteria and pathogens
of numerous plants. Flavescence dorée phytoplasma (FDp) causes severe damage to viticultural
production in Europe and is a quarantine pathogen in EU. In this study, symptomatic and
asymptomatic grapevine (Vitis vinifera L.) var. ‘Pinot gris’ leaves were sampled at three time points
through the development of infection. FDp was detected by using triplex real-time PCR and
genotyped by sequencing and phylogenetic analysis of map gene which identified two genotypes,
M38 and M54. By using spectrophotometric and chromatographic (RP-HPLC, GC-MS) analyses,
impact of two FDp genotypes on grapevine physiological parameters and phenolic metabolism was
evaluated through the development of infection. Genotype M54 impacted physiological parameters
less severely, while maintaining higher titre than M38. Correlation analysis revealed that changes
in physiological parameters could be linked to FDp titre. Content of all main branches of phenolic
metabolism and most individual compounds had similar trends, pointing to the general induction
of the stem phenolic biosynthetic pathway by FDp infection, particularly in the last time point.
Analysed phytoplasma genotype-dependant changes to grapevine physiology provide data on
certain targets of FDp in grapevine and could assist the identification of potential specific effectors
of this phytoplasma.

• Keywords: efektori; FDp; fenolni spojevi; interakcije biljka-patogen
• Keywords (english): effectors; FDp; phenolics; plant-pathogen interactions
• Language: croatian
• URN:NBN: urn:nbn:hr:217:743644
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-09-26
• Created on: 2022-10-20 12:30:34