Ekspresija gena potaknuta signalizacijom Toll-like receptora 2 u kroničnoj fazi oporavka nakon ishemijske ozljede mišjeg mozga

master's thesis

• Title (english): Expression of genes involved in TLR2 signaling pathway during the chronic phase of recovery after ischemia in mouse brain
• Author: Lora Krmpotić
• Mentor: Srećko Gajović (mentor)
• Committee member: Ivana Ivančić Baće (predsjednik povjerenstva)
• Committee member: Srećko Gajović (član povjerenstva)
• Committee member: Jasna Lajtner (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2011-07-11, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Moždani udar godišnje pogodi 15 milijuna ljudi diljem svijeta i vodeći je uzrok invaliditeta u odraslih. Ishemijski moždani udar nastaje začepljenjem arterije u mozgu, a kao posljedica nastaje hiperfuzija koja vodi ekscitotoksičnosti, oksidativnom stresu, disfunkciji krvno-moždane barijere, te aktivaciji post-ishemijske upale. Cilj ovog rada bio je pokazati kako se ekspresija gena koji sudjeluju u TLR2 signalnom putu mijenja u kroničnoj fazi reperfuzije vremenskog razdoblja od 72h do 1 mjesec. Miševima je začepljena središnja moždana arterija tijekom 1 sata, izoliran im je mozak i izmjerena razina ekspresije gena kvantitativnom lančanom reakcijom polimeraze. Rad je pokazao da se ekspresija gena aktivatora TLR2 signalnog puta smanjila, a inhibitora povećala u TLR2 KO miševa u odnosu na divlji tip. Regulacija gena u TLR2 signalizaciji mogla bi biti ključ za ublažavanje štete nastale ishemijskim moždanim udarom.

Abstract (english)

15 million people a year worldwide are afflected with stroke which is the main cause of disability in adults. Ischemic stroke is caused by blockage of blood flow in the brain, which subsequently causes hyperfusion, excitotoxicity, oxydative stress, damage of blood-brain barrier and postischemic inflammation. The aim of the thesis was to show how the expression of TLR2 signaling pathway genes changes during the chronic phase of recovery, 72 h to 1 month after induced ischemia in mouse brain. Middle cerebral artery occlusion (MCAO) was performed on mice for induction of ischemia and qPCR to measure the relative expression of selected genes. The results showed that the expression of pathway activators decreased and inhibitors increased in TLR2 KO relative to WT, in chronic phase of reperfusion. Regulation of these genes in TLR2 signaling pathway could prove to be paramount in attenuating damage caused by ischemic stroke.

• Keywords: TLR2; moždani udar; miš; ishemija; upala
• Keywords (english): TLR2; stroke; mouse; ischemia; inflammation
• Language: croatian
• URN:NBN: urn:nbn:hr:217:616858
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-03 09:08:17