Fitokemijski sastav i antioksidacijski potencijal latica odabranih cvjetnica

master's thesis

• Title (english): Phytochemical content and antioxidant potential of petals and tepals of selected flowering plants
• Author: Ana Vukres
• Mentor: Ivana Šola (mentor)
• Committee member: Ivana Šola (predsjednik povjerenstva)
• Committee member: Ivana Maguire (član povjerenstva)
• Committee member: Petra Peharec Štefanić (član povjerenstva)
• Committee member: Renata Šoštarić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2022-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Biljke su zauzele različite aspekte našega života, koristimo ih u kozmetici, medicini, kao
začine i hranu, kao bojila te u industriji ulja. Industrijska je praksa upotrebljavati određeni dio
biljke, dok se ostali dijelovi uglavnom zanemare i ne iskorištavaju. S ekološkog i
ekonomskog aspekta, takav način „djelomičnog“ iskorištavanja biljaka nije održiv. Cilj ovog
diplomskog rada bio je usporediti kvalitativni i kvantitativni sastav bioaktivnih spojeva, te
antioksidacijski potencijal etanolnih ekstrakata tepala/latica šafrana, šumskog sljeza, pravog
duhana, suncokreta i ljekovitog nevena kako bi se doprinijelo boljem razumijevanju
biopotencijala ovog biljnog materijala. Spektrofotometrijski je određen udio različitih skupina
bioaktivnih spojeva, te antioksidacijski kapacitet ekstrakata. Ekstrakti tepala šafrana sadrže
značajno veći udio ukupnih fenolnih spojeva, hidroksicimetnih kiselina, flavonola i
proantocijanidina u odnosu na ostale analizirane uzorke. 70%-tni etanolni ekstrakt duhana
sadrži više ukupnih flavonoida u odnosu na ostale istraživane cvjetnice. Najveći udio ukupnih
flavonoida u 40%-tnom etanolu imali su ekstrakti latica suncokreta. Značajno veći udio
karotenoida zabilježila sam kod ekstrakata latica nevena i suncokreta. U sve tri korištene
metode mjerenja antioksidacijskog kapaciteta u ekstraktima s 40%-tnim etanolom latice
suncokreta i sljeza imale su najviše vrijednosti, dok su u ekstraktima sa 70%-tnim etanolom
latice suncokreta bile najučinkovitije. Na temelju ovoga zaključujem da su latice suncokreta i
sljeza bogatije antioksidansima od ostalih uzoraka.

Abstract (english)

Plants have occupied different aspects of our life, we use them in cosmetics, medicine, as
spices and food, as dyes and in oil the industry. Most of these plants are known for one of
their plant organs, and these are generally not flowers, petals and tepals. It is industrial
practice to use a certain part of the plant while the other parts are mostly ignored and not
used. From an ecological and economic point of view, „partial“ exploitation of plants is not
sustainable. The aim of this thesis was to compare the qualitative and quantitative
composition of bioactive compounds, and the antioxidant potential of ethanolic extracts of
tepals/petals of saffron, common mallow, real tobacco, sunflower and medicinal calendula in
order to contribute to a better understanding of the biopotential of this plant material. The
proportion of different groups of bioactive compounds and the antioxidant capacity of the
extracts were determined spectrophotometrically. Saffron tepal extracts contained a
significantly higher proportion of total phenolic compounds, hydroxycinnamic acids,
flavonols and proanthocyanidins compared to other analyzed samples. The 70% ethanol
extract of tobacco contained more total flavonoids compared to other investigated flowering
plants. Sunflower petal extracts had the highest proportion of total flavonoids in 40% ethanol.
I noted a significantly higher proportion of carotenoids in the extracts of marigold and
sunflower petals. In all three used methods of measuring antioxidant capacity, sunflower and
common mallow petals had the highest values in extracts with 40% ethanol, while sunflower
petals were the most effective in extracts with 70% ethanol. Based on this, I conclude that
sunflower and common mallow petals are richer in antioxidants than other samples.

• Keywords: biljni metaboliti; fenolne kiseline; flavonoidi; spektrofotometrija; šećeri
• Keywords (english): flavonoids; phenolic acids; plant metabolites; spectrophotometry; sugars
• Language: croatian
• URN:NBN: urn:nbn:hr:217:874263
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2024-09-29
• Created on: 2022-10-25 11:06:30