Učinci prenatalne izloženosti alpha cipermetrinu na endokrinu disrupciju, razvoj i reprodukciju ženki Wistar štakora

master's thesis

• Title (english): Effects of prenatal alpha-cypermethrin exposure on endocrine disruption, development and reproduction of female Wistar rats
• Author: Anja Bošnjaković
• Mentor: Anja Katić (mentor)
• Mentor: Domagoj Đikić (komentor)
• Committee member: Domagoj Đikić (predsjednik povjerenstva)
• Committee member: Anamaria Štambuk (član povjerenstva)
• Committee member: Duje Lisičić (član povjerenstva)
• Committee member: Jasna Lajtner (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2022-09-28, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Piretroidi su sintetski spojevi, pesticidnog djelovanja, dobiveni derivacijom prirodnih insekticida
piretrina sadržanih u piretrumu. Budući da je dokazano da piretroidi mogu djelovati kao endokrini
disruptori, cilj ovog rada bio je istražiti utjecaj prenatalne peroralne izloženosti piretroidu αcipermetrinu na parametre endokrine disrupcije reprodukcije majki te parametre endokrine disrupcije
razvoja i reprodukcije ženskih potomaka F1 generacije. Skotne Wistar štakorice izlagane su αcipermetrinu u dozama od 1, 10 i 19 mg/kg tj. m./dan (1 mL/dan, per os) od 6. dana graviditeta do okota.
Usporedno su uspostavljene skupine negativne kontrole (voda), kontrole otapala (kukuruzno ulje) i
pozitivne kontrole (dietilstilbestrol, 10 µg/kg tj. m./dan). Parametri endokrine disrupcije reprodukcije
praćeni su u majki štakorica pri okotu, a u ženskih potomaka F1 generacije praćeni su parametri
endokrine disrupcije razvoja i reprodukcije do puberteta sukladno odgovarajućim OECD protokolima.
Tijekom gestacije izmjerene su tjelesne mase majki i potrošnja hrane. Određene su razine steroidnih
hormona u serumu ženskih potomaka u pubertetu imunokemijskom metodom ELISA Peroralna
izloženost α-cipermetrinu tijekom gestacije ne utječe na reprodukciju majki i na razvoj i reprodukciju
ženskih potomaka F1 generacije, kao ni na razine steroidnih hormona u pubertetu.

Abstract (english)

Pyrethroids are synthetic compounds, with pesticidal action, obtained by derivation of natural pyrethrin
insecticides contained in pyrethrum. Since it has been proven that pyrethroids can act as endocrine
disruptors, this work aimed to investigate the effects of prenatal oral exposure to the pyrethroid αcypermethrin on the endocrine disruption parameters of maternal reproduction and the endocrine
disruption parameters of F1 female offspring development and reproduction. Pregnant Wistar rats
were exposed to α- cypermethrin in doses of 1, 10, and 19 mg/kg b.w./day (1 mL/day, per os) from the
6
th day of pregnancyuntil delivery. Groups of negative control (water), solvent control (corn oil), and
positive control (diethylstilbestrol, 10 µg/kg b.w./day) were established concurrently. The parameters
of endocrine disruption of reproduction were monitored in mothers at delivery, and in F1 female
offspring the parameters of endocrine disruption of development and reproduction were monitored
until puberty following the appropriate OECD protocols. During gestation, maternal body mass and
food consumption were measured. Levels of steroid hormones in the serum of pubertal female offspring
were determined using immunochemical method ELISA. Oral exposure to α-cypermethrin during
pregnancy does not affect the reproduction of mothers and the development and reproduction of F1
female offspring as well as the levels of steroid hormones in puberty.

• Keywords: piretroid; endokrini disruptor; anogenitalni razmak; ulazak u pubertet; steroidni hormoni; potomci štakora
• Keywords (english): pyrethroid; endocrine disruptor; anogenital distance; onset of puberty; steroid hormones; rat offspring
• Language: croatian
• URN:NBN: urn:nbn:hr:217:006092
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-10-25 12:20:08