Genotoksični učinak karbendazima u miša Swiss albino

master's thesis

• Title (english): Genotoxic effect of pesticide Carbendazim in mice Swiss albino
• Author: Josip Parazajder
• Mentor: Vesna Benković (mentor)
• Committee member: Vesna Benković (predsjednik povjerenstva)
• Committee member: Ivančica Ternjej (član povjerenstva)
• Committee member: Alan Moro (član povjerenstva)
• Committee member: Danijel Orešić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2011-07-08, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

U današnje vrijeme upotreba pesticida za suzbijanje različitih štetnika u proizvodnji hrane je neizbježna. Nažalost, pesticidi koji zaostaju u gotovoj hrani mogu imati štetni utjecaj na zdravlje potrošača. Jedan od takvih široko upotrebljavanih pesticida je metil 2-benzimidazol karbamat, poznat kao i karbendazim, fungicid iz skupine benzimidazolnih spojeva čiji je mehanizam djelovanja inhibicija diobenog vretena za vrijeme stanične diobe. Ovo istraživanje je provedeno s ciljem procjene genotoksičnog utjecaja karbendazima na Swiss miša. Grupe od pet muških miševa i pet ženskih miševa su svakodnevno oralno tretirane NOAEL dozom karbendazima (20 mg/kg tjelesne težine) tijekom 28 dana. Za procjenu genotoksičnog utjecaja korišteni su mikronukleus test in vivo i alkalni komet test. Komet test je proveden na stanicama krvi, limfnih čvorova, slezene i timusa, a mikronukleus test na retikulocitima periferne krvi. Primjenjena NOAEL doza karbendazima tijekom 28 dana kod muških miševa uzrokovala je visoku razinu oštećenja molekule DNA što je rezultiralo istovremenim značajnim povećanjem sva tri parametra kometa u stanicama krvi i timusa. Vidljivo je da postoje razlike utjecaja karbendazima na oštećenje molekule DNA između spolova, odnosno karbendazim uzrokuje intenzivnija oštećenja DNA kod muţjaka nego kod ženki. Karbendazim je inducirao značajno povećanje učestalosti mikronukleusa kod oba spola.

Abstract (english)

In modern times the usage of pesticides to repel pests in food production is a necessity. Unfortunately, the pesticide residues in food can have a negative impact on consumers health. One of those widely used pesticides is metil 2-benzimidazole carbamate, also known as carbendazim, a benzimidazole fungicide whose mode of action is the inhibition of the spindle function during the cell division. This expirement was conducted in order to evaluate the genotoxic effect of carbendazim on the Swiss mouse. Groups of five male and five female mice were oraly given NOAEL dosage of carbendazim (20 mg/kg of body weight) during te period of 28 days. To evaluate the genotoxic effect micronucleus test in vivo and the single cell gel electrophoresis assay (comet assay) were used. The comet assay was conducted on blood, lymph node, spleen and thymus cells while the micronucleus test was conducted on the peripheral blood reticulocytes. The administered NOAEL dose of carbendazim during the 28 days caused high levels of DNA damage in male mice resulting in simultaneous significant increases of all three comet parameters in blood and thymus cells. Results show that there are differences of impact of carbendazim on DNA damage between sexes, that is carbendazim causes more intense DNA damage in male mice than in female mice. Carbendazim induced significant increase in the frequency of micronuclei in both sexes.

• Keywords: genotoksičnost; komet-test; mikronukleus; Swiss-miš
• Keywords (english): arbendazim; genotoxicity comet-assay; micronucleus; Swiss-mice
• Language: croatian
• URN:NBN: urn:nbn:hr:217:758340
• Study programme: Title: Enviromental Sciences; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra struke znanosti o okolišu (magistar/magistra struke znanosti o okolišu)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-03 09:41:32