ULOGA SEKRETORNIH PROTEINA U RAZVOJU IZVANMATERIČNE MIGRACIJE ENDOMETRIJA

doctoral thesis

• Title (english): THE ROLE OF SECRETORY PROTEINS OF EXTRAUTERINE MIGRATION OF ENDOMETRIUM
• Author: Alenka Višnić
• Mentor: Dubravko Barišić (mentor); MBZ: 141010
• Mentor: Neda Smiljan Severinski (komentor)
• Committee member: Vesna Benković (predsjednik povjerenstva)
• Committee member: Petra Korać (član povjerenstva)
• Committee member: Dinka Pavičić Baldani (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2022-06-03, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 577 - Biochemistry. Molecular biology. Biophysics

Abstract

Izvanmaterična migracija endometrija je kronična upalna bolest karakterizirana pojavom i
rastom endometrija izvan maternice. Etiopatogeneza nije u potpunosti razjašnjena. Patološki
procesi koji se pri tome događaju su invazija, adhezija, proliferacija, angiogeneza i poremećena
imunost. Moguće je da procesi invazije i proliferacije uzrokuju povećanu produkciju određenih
proteina, a izlučuju se urinom. Ovim istraživanjem željelo se proteomskim tehnikama (metoda
Bradford, jednodimenzionalna elektroforeza, dvodimenzionalna elektroforeza, ELISA, western
blot i masena spektrometrija) odrediti proteine u urinu koji se izlučuju u povećanoj
koncentraciji kod ekstrauterine migracije endometrija. Utvrđena je povišena koncentracija
proteina koji sudjeluju u apoptozi, imunosnim, vaskularnim, antioksidativnim, proliferacijskim
i adhezijskim reakcijama kao i korelacija koncentracije proteina CYFRA 21-1 i stupnja
ekstrauterine migracije endometrija. Protein CYFRA 21-1 i drugi proteini u urinu pojačano se
izlučuju u ekstrauterinoj migraciji endometrija.

Abstract (english)

Extrauterine migration of endometrium is a chronic gynecological inflammatory disease
characterized with appearing and fluorishing of endometrium outside the uterine cavity.
Ethiopathogenesis is not clearly understood. Ongoing pathological processes are invasion,
adhesion, proliferation, angiogenesis and immune disorders. There is a possibility for increased
production of proteins caused by proliferation and invasion. It's excreted in urine. The purpose
of the study is to determine increased levels of proteins in urine during extrauterine migration
of endometrium using proteomic techniques (Bradford method, one-dimensional
electrophoresis, two-dimensional electrophoresis, ELISA, western blot and mass
spectrometry). Increased production of involved in apoptosis, immune, vascular, antioxidant,
proliferative and adhesive reactions protein as well as the correlation between CYFRA 21-1
protein increased the concentration and degree of extrauterine migration of endometrium.
Protein CYFRA 21-1 and other proteins in urine are enhanced production in the state of
extrauterine migration of endometrium.

• Keywords: ekstrauterina migracija endometrija; citokeratin 19-9; CYFRA 21-1; proteini; urin
• Keywords (english): extrauterine migration of endometrium; cytokeratin 19-9; cyphra 21-1; proteins; urine
• Language: croatian
• URN:NBN: urn:nbn:hr:217:012534
• Promotion: 2022
• Study programme: Title: Biology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2024-06-03
• Created on: 2022-11-21 11:33:45