VALIDACIJA MJERENJA FRAKCIONIRANE TEMPERATURE IZDAHA TE NJEZINA POVEZANOST S RAZLIČITIM ENDOGENIM, EGZOGENIM I OKOLIŠNIM ČIMBENICIMA U ZDRAVIH ISPITANIKA

doctoral thesis

• Title (english): VALIDATION OF THE FRACTIONAL EXHALED BREATH TEMPERATURE MEASUREMENT AND ITS ASSOCIATION WITH DIFFERENT INTRINSIC, EXTRINSIC AND ENVIRONMENTAL FACTORS IN HEALTHY SUBJECTS
• Author: Ivana Huljev Šipoš
• Mentor: Marina Labor (mentor)
• Mentor: Kristian Vlahoviček (komentor)
• Committee member: Snježana Židovec-Lepej (predsjednik povjerenstva)
• Committee member: Domagoj Đikić (član povjerenstva)
• Committee member: Davor Plavec (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2022-07-14, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 57 - Biological sciences. Physical anthropology. Biodiversity

Abstract

Temperatura izdahnutog zraka (EBT) poznata je kao biomarker upale i prokrvljenosti
dišnih putova. U istraživanju je mjerena temperatura različitih frakcija izdahnutog zraka, fEBT
(iz perifernih i centralnih dišnih putova). Svrha ovog istraživanja bila je validirati tu metodu
kako bi se utvrdile referentne vrijednosti te utjecaj različitih čimbenika na njih.
Hipoteze istraživanja bile su da je fEBT iz centralnog dišnog sustava niži i varijabilniji
u odnosu na onaj iz periferne frakcije te da je statistički značajno povezan s tjelesnom
temperaturom mjerenom na specifičnim točkama. Ova presječna studija obuhvaćala je 55
odraslih osoba kojima je opetovano mjeren fEBT, dva dana za redom, koristeći FractAir uređaj.
Mjereni su i bazalni metabolizam, razina fizičke aktivnosti, udaljenost od glavne prometnice,
vanjska i sobna temperatura, tlak i vlaga u zraku, KKS, CRP, plućne funkcije, kumulirani EBT
i tjelesne temperature.
Rezultati su osnovnu hipotezu potvrdili, dok je sekundarna odbačena. Također, pokazali
su da fEBT nije povezan s tjelesnom temperaturom (p > 0,05 za sve) te da je ponovljiv tijekom
dvaju uzastopnih dana. Svi su fEBT-ovi korelirali s temperaturom sobnog zraka (p < 0,01). U
multivarijatnoj analizi nisu nađene povezanosti fEBT s ostalim osobnim i vanjskim
čimbenicima. Pri temperaturi sobe od 22 °C fiziološke vrijednosti temperature prve frakcije
bile su 23,481 ± 3,150 °C, druge frakcije 26,114 ± 4,024 °C, a treće frakcije 28,216 ± 3,321 °C.

Abstract (english)

Exhaled air temperature (EBT) is known as a biomarker of inflammation and airway
blood flow. The temperature of different fractions of exhaled air, fEBT (from peripheral and
central airways) was measured in the research. The purpose of this study was to validate this
method in order to determine the reference values and the influence of different factors on them.
The research hypotheses were that fEBT from the central respiratory system was lower
and more variable than the one from the peripheral and that it was statistically related to body
temperature measured at specific points. This cross-sectional study included 55 adults in which
the fEBT was repeatedly measured, two days in a row, using a FractAir device. Basal
metabolism, level of physical activity, distance from the main road, outdoor and room
temperature, air pressure and humidity, KKS, CRP, lung function, accumulated EBT and body
temperatures were also measured.
The results confirmed the primary hypothesis, while the secondary was rejected. They
also showed that fEBT was not associated with body temperature (p> 0.05 for all) and was
recurrent for two consecutive days. All fEBTs correlated with room temperature (p <0.01). No
associations of fEBT with other personal and external factors were found in the multivariate
analysis. At room temperature of 22 ° C, the physiological values of the temperature of the first
fraction were 23.481 ± 3.150 ° C, the second 26.114 ± 4.024 ° C, and the third fraction 28.216
± 3.321 ° C.

• Keywords: disanje; temperatura; EBT; metabolizam; dišni sustav
• Keywords (english): breathing; temperature; EBT; metabolism; respiratory tract
• Language: croatian
• URN:NBN: urn:nbn:hr:217:856583
• Promotion: 2022
• Study programme: Title: Biology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-11-22 11:46:50