Učinak krizina u prevenciji razvoja osteoporoze štakora

master's thesis

• Title (english): The effect of chrysin in preventing the development of osteoporosis in rats
• Author: Anita Šamonik
• Mentor: Nada Oršolić (mentor)
• Committee member: Nada Oršolić (predsjednik povjerenstva)
• Committee member: Alan Moro (član povjerenstva)
• Committee member: Mladen Kerovec (član povjerenstva)
• Committee member: Danijel Orešić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2011-03-17, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Osteoporoza je bolest karakterizirana gubitkom koštane gustoće i propadanjem mikroarhitekture kostiju, što rezultira povećanim rizikom za prijelom. Etiologija osteoporoze je raznolika, a postmenopauzalna osteoporoza je najčešća. Danas se u terapiji koriste brojne skupine lijekova: antiresorptivni lijekovi, estrogeni, selektivni modulatori estrogenskih receptora, bisfosfonati, paratireoidni hormon, stroncijev renalat. Zbog nekih neželjenih učinaka kao prirodna alternativa nameću se fitoestrogeni. Cilj ovog istraživanja bio je istražiti mogući zaštitni učinak krizina na retinoičnom kiselinom prouzročenu osteoporozu u štakora. Oralno davanje krizina u dozi od 100 mg kg-1 i retinoične kiseline u dozi od 80 mg kg-1 štakorima za period od 15 dana rezultira u značajnom povećanju sadržaja suhog materijala kosti, sadržaja kalcija i fosfora u štakora; krizin je spriječio osteoporozu prouzročenu retinoičnom kiselinom. Nadalje, krvni uzorci su sakupljeni nakon obrade štakora istraživanim tvarima za analizu oštećenja DNA i broja mikronukleusa u retikulocitima periferne krvi pomoću standardnog komet testa i mikronukleus testa. Rezultati genotoksičnog istraživanja ukazuju da je krizin siguran za uporabu i da ne prouzrokuje nikakva genotoksična oštećenja. Iz dobivenih rezultata je vidljivo da krizin ima veliki potencijal u sprječavanju osteoporoze in vivo.

Abstract (english)

Osteoporosis is a disease characterised by the loss of bone density and deterioration of bone micro architecture, leading to an increased risk of fracture. The aetiology of osteoporosis is varied whereby postmenopausal osteoporosis is the most common form. Several classes of drugs are available for the treatment of osteoporosis: antiresorptive drugs, oestrogen, selective oestrogen receptor modulators, bisphosphonates, parathyroid hormone, and strontium ranelate. Due to the adverse effects of these drugs, a natural alternative offered in the form of phytoestrogens is of interest. The present study was designed to investigate the possible protective effect of preparations of chysin against retinoic acid -induced osteoporosis in rats. Oral administration of chrysin at doses of 100 mg kg-1 and retinoic acid at dose of 80 mg kg-1 to rats for a period of 15 days resulted in a significant increase the content of bone ash, calcium, and phosphorus content in rats; chrysin prevented osteoporosis induced by retinoic acid. In addition, blood samples were collected after treatment of rats with test components to analyze DNA damage and number of micronucleus in reticulocytes of peripheral blood by standard comet assays and micronucleus test. The results from the genotoxicity studies suggest chrysin is safe for consumption with no observed adverse effect on peripheral lymphocytes. These outcomes suggest that chrysin offer a potential in the management of osteoporosis in vivo.

• Keywords: osteoporoza; krizin; retinoična kiselina; genotoksičnost mikronukleus-test
• Keywords (english): osteoporosis; chrisyn; retinoic acid; genotoxicity micronucleus test
• Language: croatian
• URN:NBN: urn:nbn:hr:217:402746
• Study programme: Title: Enviromental Sciences; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra struke znanosti o okolišu (magistar/magistra struke znanosti o okolišu)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-03 10:22:08