Genetic diversity and phylogeography of fishes of the genus Gobio (Gobionidae) and their ectoparasites of the genus Gyrodactylus (Platyhelminthes: Monogenea) in Croatia and Austria

master's thesis

• Title (croatian): Genska raznolikost i filogeografija riba roda Gobio (Gobionidae) i njihovih ektoparazita roda Gyrodactylus (Platyhelminthes: Monogenea) u Hrvatskoj i Austriji
• Author: Martina Topić
• Mentor: Stephan Koblmüller (mentor)
• Mentor: Zoran Marčić (komentor)
• Committee member: Zoran Marčić (predsjednik povjerenstva)
• Committee member: Sandra Hudina (član povjerenstva)
• Committee member: Duje Lisičić (član povjerenstva)
• Committee member: Mirela Sertić Perić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2023-02-21, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Freshwater fishes of the genus Gobio are widely distributed in Eurasia, where they show great
phenotypic similarity between different species, but also considerable intraspecific morphological
variability. The genetic research so far showed that there is also a remarkable genetic diversity
present within the genus, and that Gobio is genetically very diverse in the Danube River drainage.
This research aimed at exploring the genetic diversity and phylogeography of Gobio in Croatia and
Austria through DNA barcoding, and additionally investigating the diversity of ectoparasites of the
genus Gyrodactylus which reside on Gobio, potentially enhancing our understanding of the host’s
phylogeographic patterns through insights from the parasites’. The results of this research
uncovered the presence of the new mitochondrial lineage of Gobio in Croatia, which is closely
related to other southeast European Gobio species, and a distinct phylogeographic pattern within
the genus in Austria and Croatia. The parasites of the genus Gyrodactylus also showed great
diversity, with several new lineages uncovered, but without a clear phylogeographic structure.
Furthermore, the phylogenetic analysis indicates that Gyrodactylus colonized fishes of the genus
Gobio in the Danube River drainage twice independently.

Abstract (croatian)

Slatkovodne ribe roda Gobio široko su rasprostranjene u Euroaziji, gdje pokazuju veliku fenotipsku
sličnost među vrstama, ali također značajnu intraspecijsku morfološku varijabilnost. Genska
istraživanja dosad provedena na ovim ribama pokazala su da unutar roda postoji velika genska
raznolikost i da je ta raznolikost izražena kod pripadnika roda Gobio u dunavskom slijevu. Cilj
ovog istraživanja bio je istražiti gensku raznolikost i filogeografiju roda Gobio u Hrvatskoj i
Austriji primjenom DNA barkodiranja te istražiti raznolikost ektoparazita roda Gyrodactylus, koji
se mogu pronaći na spomenutim ribama, s ciljem boljeg razumijevanja filogeografskih uzoraka
domaćina uvidom u filogeografske uzorke parazita. Rezultati ovog istraživanja otkrivaju u
Hrvatskoj dosad nepoznatu mitohondrijsku liniju riba roda Gobio, koja je u bliskom srodstvu s
drugim vrstama roda Gobio iz jugoistočne Europe te da je prisutan filogeografski uzorak među
pripadnicima ovog roda u Austriji i Hrvatskoj. Paraziti roda Gyrodactylus su također pokazali
veliku gensku raznolikost te su otkrivene nove genske linije tog roda, međutim nije utvrđeno
postojanje jasne filogeografske strukture. Ipak, filogenetska analiza ukazuje na to da su paraziti tog
roda dvaput neovisno kolonizirali ribe roda Gobio u dunavskom slijevu.

• Keywords: gudgeons; biodiversity; DNA barcoding; taxonomy; hybridization
• Keywords (croatian): krkuše; bioraznolikost; DNA barkodiranje; taksonomija; hibridizacija
• Language: english
• URN:NBN: urn:nbn:hr:217:064026
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-02-21
• Created on: 2023-02-28 10:54:26