Sezonska promjenjivost sastava mikrobne zajednice slane močvare Palud

master's thesis

• Title (english): Seasonal variability of microbial communities in the Palud salt swamp
• Author: Marin Vukušić
• Mentor: Paolo Paliaga (mentor)
• Mentor: Tomislav Ivanković (komentor)
• Committee member: Tomislav Ivanković (predsjednik povjerenstva)
• Committee member: Sandra Hudina (član povjerenstva)
• Committee member: Ivana Maguire (član povjerenstva)
• Committee member: Sunčica Bosak (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2023-02-22, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Iako su močvare stoljećima promatrane kao bezvrijedna i nepotrebna područja u zadnjim se desetljećima
taj stav postepeno promijenio. Danas su močvarni ekosustavi prepoznati kao vrlo vrijedna staništa zbog
izuzetno visoke produktivnosti te zbog bogatstva vrsta od najsitnijih mikroorganizama pa sve do biljaka
i životinja. Cilj rada bio je istražiti sezonsku dinamiku autohtone mikrobne zajednice i fekalnih bakterija
u posebnim hidrografskim uvjetima voda slane močvare Palud i usporediti je sa morskom mikrobnom
zajednicom u neposrednoj blizini močvare. Područje posebnog ornitološkog rezervata Palud – Palù od
posebnog je značaja za sve organizme koje žive na tome području, a močvara koja se nalazi u samome
središtu rezervata je specifičan ekosustav na području sjevernog Jadrana. Uzorkovanje se je izvodilo na
9 prethodno određenih postaja, na području posebnog ornitološkog rezervata Palud – Palù, a održalo se
je 7 puta u periodu od kraja srpnja 2021., do svibnja 2022. Određena je sanitarna kakvoća vode te
brojnost autohtone mikrobne zajednice u močvari, moru te u močvarnom kanalu koji povezuje močvaru
s morem. Također je napravljena i statistička analiza podataka radi prikaza korelacije između
sezonalnosti i mjerenih abiotičkih čimbenika, kao i grupacija postaja ovisno o poziciji uzorkovanja i
ovisno o prisutnosti indikatorskih mikroorganizama ili autohtone mikrobne zajednice. Tijekom toplijih
mjeseci (ljeto 2021. i proljeće 2022.) koncentracija indikatora fekalnog onečišćenja, ali i brojnost
autohtone mikrobne zajednice je bila veća nego tijekom hladnijih mjeseci (jesen 2021. i zima 2022.).
Dobiveni rezultati uspoređeni su s prosječnim podacima za sjeverni Jadran dostupnim u literaturi, kao i
s dugoročnim prosjecima podataka za postaju RV001 na 1 NM zapadno od Rovinja. Rezultati su
pokazali da hidrografski parametri u močvari imaju velike sezonske oscilacije, ali i da morske mijene
imaju izuzetno važnu ulogu u funkcioniranju mikrobne zajednice i čitavog ekosustava.

Abstract (english)

Although wetlands have been viewed as worthless and unnecessary areas for centuries, this attitude has
gradually changed in recent decades. Today, wetland ecosystems are recognized as very valuable
habitats due to their extremely high productivity and the richness of species, from the smallest
microorganisms to plants and animals. The aim of this research is to investigate the seasonal dynamics
of the indigenous microbial community and fecal bacteria in the special hydrographic conditions of the
waters of the Palud salt marsh and compare it with the marine microbial community in the immediate
vicinity of the marsh. The area of the special ornithological reserve Palud - Palù is of special importance
for all organisms that live in that area, and the marsh located in the very center of the reserve is a specific
ecosystem in the area of the northern Adriatic. Sampling was carried out at 9 previously determined
stations, in the area of the special ornithological reserve Palud - Palù and took place 7 times in the period
from the end of July 2021 to May 2022. The sanitary quality of the water and the abundance were
determined autochthonous microbial communities in the marsh, the sea and in the marsh channel that
connects the marsh with the sea. A statistical analysis of the data was also made to show the correlation
between seasonality and measured abiotic factors, as well as the grouping of stations depending on the
sampling position and depending on the presence of indicator microorganisms or autochthonous
microbial communities. During the warmer months (summer 2021 and spring 2022), the concentration
of fecal pollution indicators, as well as the abundance of the autochthonous microbial community, was
expected to be higher than during the colder months (autumn 2021 and winter 2022). The obtained
results were compared with average data for the northern Adriatic available in the literature, as well as
with long-term data averages for station RV001 at 1 NM west of Rovinj. The results showed that
hydrographic parameters in the marsh have large seasonal oscillations, but also that sea changes play an
extremely important role in the functioning of the microbial community and the entire ecosystem.

• Keywords: indikatori fekalnog onečišćenja; heterotrofni prokarioti; heterotrofni nanoflagelati; cijanobakterije
• Keywords (english): indicators of fecal pollution; heterotrophic prokaryotes; heterotrophic nanoflagellates; cyanobacteria
• Language: croatian
• URN:NBN: urn:nbn:hr:217:082548
• Study programme: Title: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-02-22
• Created on: 2023-02-28 11:01:55