DJELOVANJE STROBILURINSKIH FUNGICIDA NA BIOKEMIJSKE, STANIČNE I POPULACIJSKE BIOMARKERE ENHITREJA VRSTA Enchytraeus crypticus i Enchytraeus albidus

doctoral thesis

• Title (english): EFFECT OF STROBILURIN FUNGICIDES ON BIOCHEMICAL, CELLULAR AND POPULATION BIOMARKERS OF Enchytraeus crypticus AND Enchytraeus albidus
• Author: Marija Kovačević
• Mentor: Branimir Hackenberger Kutuzović (mentor)
• Committee member: Göran Klobučar (predsjednik povjerenstva)
• Committee member: Ivana Maguire (član povjerenstva)
• Committee member: Maja Šegvić Klarić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2023-02-27, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 57 - Biological sciences. Physical anthropology. Biodiversity

Abstract

Enhitreje su bliski srodnici gujavica te modelni organizmi u ekotoksikološkim istraživnjima tla.
Iako imaju važnu ulogu u ekološkim sustavima tla, djelovanje pojedinih zagađivala, posebice
strobilurinskih fungicida nedovoljno je istraženo. Stoga je cilj ovog doktorskog rada nadopuniti
postojeća istraživanja novim znanjima o utjecaju strobilurinskih fungicida na enhitreje vrsta E.
crypticus i E. albidus. Razvijena je metoda za mjerenje promjena aktivnosti mehanizma
multiksenobiotske rezistentnosti čime se dokazala prisutnost ovog mehanizma kod enhitreja i
mogućnost njegova korištenja kao biomarkera zagađenja. Nadalje, uočeno je negativno
djelovanje strobilurinskih fungicida na preživljavanje, reprodukciju i uspjeh izlijeganja
enhitreja. Prikupljena inicijalna znanja o djelovanju aktivne tvari i različitih pripravaka na
biokemijske i stanične biomarkere ukazuju na moguće mehanizme djelovanja strobilurinskih
fungicida naglašavajući važnost sveobuhvatnog pristupa.

Abstract (english)

Enchytraeids are close relatives of earthworms and model organisms of soil ecotoxicology.
Although they play an important role in soil ecosystems, the effects of certain pollutants,
especially strobilurin fungicides, have not been sufficiently investigated. Therefore, the main
objective of this doctoral thesis is to supplement existing research with new knowledge about
the influence of strobilurin fungicides on the enchytraeid species E. crypticus and E. albidus.
The development of a method for measuring changes in the activity of the multixenobiotic
resistance mechanism proved the presence of this mechanism in enchytraeids and the possibility
of its use as a biomarker. Furthermore, a negative effect of strobilurin fungicides was observed
on the survival, reproduction, and hatching success of enchytraeids. Initial knowledge collected
on the effect of the active ingredient and different formulations on biochemical and cellular
biomarkers indicates the possible mechanisms of action of strobilurin fungicides, emphasizing
the importance of a comprehensive approach.

• Keywords: enhitreje; strobilurinski fungicidi; reprodukcija; oksidativni stres; energetski status
• Keywords (english): enchytraeid; strobilurin fungicides; reproduction; oxidative stress; energy status
• Language: croatian
• URN:NBN: urn:nbn:hr:217:097712
• Promotion: 2023
• Study programme: Title: Biology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-02-28 13:30:19