Raznolikost tipova vegetativne kompatibilnosti gljive Cryphonectria parasitica i genska analiza ORFA regije virusa Cryphonectria hypovirus 1 u populacijama Istre i otoka Cresa

master's thesis

• Title (english): Diversity of vegetative compatibility types of fungus Cryphonectria parasitica and genetic analysis of ORFA region of Cryphonectria hypovirus 1 in populations of Istria and Cres island
• Author: Ozren Mendaš
• Mentor: Marin Ježić (mentor)
• Committee member: Marin Ježić (predsjednik povjerenstva)
• Committee member: Anamaria Štambuk (član povjerenstva)
• Committee member: Biljana Balen (član povjerenstva)
• Committee member: Sofia Ana Blažević (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2023-02-28, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Gljiva Cryphonectria parasitica uzrokuje bolest rak kore pitomog kestena (Castanea sativa),
te je u proteklom stoljeću ugrozila šume pitomog kestena diljem Europe. Prisutnost virusa
Cryphonectria hypovirus 1 (CHV1) u populacijama gljive povezana je sa smanjenjem
virulentnosti i blažim tijekom bolesti raka kore kestena, tj. tijekom vremena dolazi do
uspostavljanja prirodne biološke kontrole bolesti. Kako bi se razumjela sama raznolikost gljive,
analizirani su tipovi vegetativne kompatibilnosti (vc) ove gljive. U ovom istraživanju utvrđena
je raznolikost istraživanih populacija pomoću Shannonovog indeksa (H') koji u populaciji
gljive otoka Cresa iznosi 1,52, a u populaciji Istre 1,33. U populaciji C. parasitica otoka Cresa
potvrđeno je šest različitih EU tipova, dok je u populaciji Buje, Istra potvrđeno pet. Također,
50% izolata gljive populacije otoka Cresa sadrži virus CHV1, dok u populaciji Istre virus nije
nađen. Računalnom analizom sekvenci virusne RNA u creskoj populaciji gljive nađeno je devet
haplotipova virusa. Analizirane sekvence virusa CHV1 imaju ukupno 39 polimorfizama u 985
nukleotida, te je potvrđeno da CHV1 u populaciji Cresa pripada talijanskom podtipu.

Abstract (english)

Fungus Cryphonectria parasitica causes the chestnut blight disease in sweet chestnut
(Castanea sativa) and has endangered the forests of sweet chestnut across Europe in the last
century. The presence of Cryphonectria hypovirus 1 (CHV1) in fungal populations is
connected to the reduction of the fungus’ virulence and a milder disease progress of chestnut
blight, or in other words, a natural biological control is established over time. In order to
understand the diversity of C. parasitica populations, an analysis of its vegetative compatibility
(vc) types has been carried out. In this research, the diversity of the analysed populations was
determined using the Shannon Diversity Index (H’), which was determined to be 1.52 in the
fungal population of the island Cres, and 1.33 in the population of Buje, Istria. In the population
of the C. parasitica of the island Cres six different EU-types were found, whereas in the
population of Istria there were five. Moreover, 50% of the fungal isolates in the population of
the island Cres were infected with the CHV1 virus, while in the population of Istria the virus
was not found. By analysing CHV1 viral sequences in the population of Cres nine CHV1
haplotypes were found. Analysed sequences of the CHV1 virus have 39 polymorphisms in 985
nucleotides in total, and it has been determined that the CHV1 in the population of Cres belongs
to the Italian subtype of the virus.

• Keywords: biljni patogen; CHV1; pitomi kesten; hipovirulencija; vegetativna kompatibilnost
• Language: croatian
• URN:NBN: urn:nbn:hr:217:145764
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-02-28
• Created on: 2023-03-08 10:53:23