Sinkronizacija i povećanje mitotske aktivnosti stanica duhana (Nicotiana tabacum L.) u suspenziji

master's thesis

• Title (english): Synchronization and increase of mitotic activity in tobacco cells (Nicotiana tabacum L.) in suspension
• Author: Tihana Čus
• Mentor: Dunja Leljak-Levanić (mentor)
• Committee member: Dunja Leljak-Levanić (predsjednik povjerenstva)
• Committee member: Tomislav Cvitaš (član povjerenstva)
• Committee member: Zdravko Dolenec (član povjerenstva)
• Committee member: Draginja Mrvoš-Sermek (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2010-09-29, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Stanična linija kultivara duhana BY-2 (Nicotiana tabacum L. cv. Bright Yellow No.2) jedna je od najčešće primjenjivanih suspenzija stanica u biljnoj biologiji. Cilj istraživanja je bio pratiti unutarstaničnu lokalizaciju proteina nepoznate funkcije iz kukuruza (ZmMAB1) tijekom staničnog ciklusa. Sinkronizacija staničnog ciklusa stanica duhana BY-2 u tekućoj podlozi postignuta je pažljivim odabirom početnog razrjeđenja, stupnja diferencijacije te perioda supkultivacije. U staničnoj kulturi, ostavljenoj dva tjedna bez presađivanja, stanice su se diferencirale te su se supkultivacijom u svježu podlogu počinjale dijeliti, a treći dan nakon presađivanja postignut je najveći mitotski indeks. Vizualizacija mikrotubula omogućavala je prepoznavanje pojedinih stadija mitoze, a postignuta je flourescentno obilježenim proteinom MAP-4. Obilježavanjem proteina kukuruza ZmMAB1 crvenim fluorescentnim proteinom uočena je njegova kolokalizacija s mikrotubulima tijekom diobe.

Abstract (english)

The cell line of tobacco cultivar BY-2 (Nicotiana tabacum L. cv. Bright Yellow No.2) is one of the most commonly used in plant cell biology. The purpose of this study was to follow the intracellular localization of protein of unknown function from maize (ZmMAB1) during the cell cycle. Synchronization of the cell cycle of tobacco BY-2 cells in the liquid medium was achieved by careful selection of initial dilution, degree of differentiation and subculturing period. In the cell culture which was left two weeks without subculturing, cells became differentiated and mature but after resubculturing in fresh medium, they began to divide, and the third day they reach the highest mitotic index. Visualization of microtubules allowed the identification of particular stages of mitosis, and that was achieved by flourescently labeled protein MAP-4. The maize protein ZmMAB1 labeled with red fluorescent protein showed its colocalization with the microtubules during division.

• Keywords: sinkronizacija; mitotski indeks; stanice Bright Yellow No.2; stanična dioba
• Keywords (english): synchronization; mitotic index; Cells Bright Yellow No.2; cell division
• Language: croatian
• URN:NBN: urn:nbn:hr:217:342402
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-03 12:41:09