Izražaj glijalnog fibrilarnog kiselog proteina (GFAP) u mozgu odraslih štakora nakon perinatalne hipoksije

master's thesis

• Title (english): Expression of the glial fibrillary acidic protein (GFAP) in the adult rat brain following perinatal hypoxia
• Author: Marta Radošević
• Mentor: Nataša Jovanov Milošević (mentor)
• Mentor: Duje Lisičić (komentor)
• Committee member: Duje Lisičić (predsjednik povjerenstva)
• Committee member: Marija Ivković (član povjerenstva)
• Committee member: Sofia Ana Blažević (član povjerenstva)
• Committee member: Domagoj Đikić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2023-01-20, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Cilj ovog pilot istraživanja je utvrditi učinak kratkotrajne perinatalne hipoksijske ozljede u
odraslih štakora na razinu izražaja GFAP-a. Korišteno je osam ženki Wistar Han (RccHan:WIST)
štakora dobi P(105) – četiri kontrolne i četiri perinatalno podvrgnute kratkotrajnoj hipoksiji.
Histološki preparati bili su podvrgnuti imunofluorescentnom bojenju korištenjem anti-GFAP
protutijela i flourescentnom Nissl bojenju. Kvalitativna analiza 20 presjeka nije pokazala promjene
u citoarhitektonici hipokampusa, dok je uočen viši intenzitet izražaja GFAP+
astrocita nakon
kratkotrajne perinatalne hipoksije. Kvantitativna analiza 20 presjeka pokazala je tendenciju k
povećanju gustoće astrociti/mm2
u polju CA1 u hipoksijskoj skupini štakora. Gustoća
astrociti/mm2
u polju CA2 ne pokazuje razlike između hipoksijske i kontrolne skupine. Izazvana
kratkotrajna perinatalna hipoksija bila je blaga i uzrokovala je suptilne promjene koje nisu
detektirane na ovom broju uzoraka. Potencijalno daljnje istraživanje povećanjem broja životinja u
eksperimentu te samim time i broja uzoraka, moglo bi pokazati je li promjena morfologije astrocita
ili je povećanje izražaja GFAP+
astrocita, razlog kvalitativnog utiska da je GFAP pozitivnih
astrocita više u hipokampusu perinatalno hipoksijsko ozlijeđenih štakora.

Abstract (english)

The aim of this pilot study was to research the effects of short-term perinatal hypoxia on
the expression of GFAP. For this purpose, eight female Wistar Han (RccHan:WIST) rats age
P(105) – four controls and four perinatally treated with short-term hypoxia, were used. Samples
were double stained using anti-GFAP antibody and Nissl stain. Qualitative analysis of 20 slices
didn't indicate any change in the cytoarchitecture of the hippocampus, while there was a slight
increase detected in the GFAP+
astrocyte signal after hypoxia. Quantitative analysis of 20 slices
shows a tendency towards an increase in density of astrocyte/mm2
in the CA1 field of the hypoxic
rat group. Density of astrocyte/mm2
in the CA2 field didn't show any difference between hypoxic
and control group. The perinatal hypoxic injury in this study was short-term and mild, and it caused
subtle changes which could not be registered on this small sample size. Future experiments, with a
bigger sample size, might show if the qualitative phenomen of more GFAP positive astrocytes in
the hippocampus of perinatal hypoxically injured rats, is due to change in astrocyte morphology or
due to an increase in GFAP expression in astrocytes.

• Keywords: hipokampus; Nissl; astrociti; hipoksijska ozljeda; Fiji/ImageJ
• Keywords (english): hippocampus; Nissl; astrocytes; hypoxic injury; Fiji/ImageJ
• Language: croatian
• URN:NBN: urn:nbn:hr:217:571659
• Project: Number: IP-2019-04-3182; Title: Moždani izvanstanični matriks u razvitku i perinatalnoj hipoksiji; Title: Brain extracellular matrix in development and in perinatal hypoxia; Acronym: BRAINECM; Leader: Nataša Jovanov Milošević; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: IP
• Project: Number: GA KK.01.1.1.01.0007; Title: Eksperimentalna i klinička istraživanja hipoksijsko-ishemijskog oštećenja mozga u perinatalnoj i odrasloj dobi, ZCI-Neuro; Leader: Miloš Judaš; Jurisdiction: Croatia; Funder: ERDF
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-01-20
• Public note: Sveučilišna potpora br. 10106-22-3116, voditeljica projekta prof. dr. sc. Nataša Jovanov- Milošević
• Created on: 2023-04-05 10:08:23