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doctoral thesis
MOLECULAR IDENTIFICATION OF FRESHWATER PATHOGENIC OOMYCETES AND DETERMINATION OF NOVEL FACTORS THAT PROMOTE THEIR SPREADING
2023. urn:nbn:hr:217:876903
Pavić, Dora
University of Zagreb
Faculty of Science
Department of Biology

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Pavić, D. (2023). MOLECULAR IDENTIFICATION OF FRESHWATER PATHOGENIC OOMYCETES AND DETERMINATION OF NOVEL FACTORS THAT PROMOTE THEIR SPREADING (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Science. Retrieved from https://urn.nsk.hr/urn:nbn:hr:217:876903

Pavić, Dora. "MOLECULAR IDENTIFICATION OF FRESHWATER PATHOGENIC OOMYCETES AND DETERMINATION OF NOVEL FACTORS THAT PROMOTE THEIR SPREADING." Doctoral thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:876903

Pavić, Dora. "MOLECULAR IDENTIFICATION OF FRESHWATER PATHOGENIC OOMYCETES AND DETERMINATION OF NOVEL FACTORS THAT PROMOTE THEIR SPREADING." Doctoral thesis, University of Zagreb, Faculty of Science, 2023. https://urn.nsk.hr/urn:nbn:hr:217:876903

Pavić, D. (2023). 'MOLECULAR IDENTIFICATION OF FRESHWATER PATHOGENIC OOMYCETES AND DETERMINATION OF NOVEL FACTORS THAT PROMOTE THEIR SPREADING', Doctoral thesis, University of Zagreb, Faculty of Science, accessed 21 January 2025, https://urn.nsk.hr/urn:nbn:hr:217:876903

Pavić D. MOLECULAR IDENTIFICATION OF FRESHWATER PATHOGENIC OOMYCETES AND DETERMINATION OF NOVEL FACTORS THAT PROMOTE THEIR SPREADING [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Science; 2023 [cited 2025 January 21] Available at: https://urn.nsk.hr/urn:nbn:hr:217:876903

D. Pavić, "MOLECULAR IDENTIFICATION OF FRESHWATER PATHOGENIC OOMYCETES AND DETERMINATION OF NOVEL FACTORS THAT PROMOTE THEIR SPREADING", Doctoral thesis, University of Zagreb, Faculty of Science, Zagreb, 2023. Available at: https://urn.nsk.hr/urn:nbn:hr:217:876903

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Metadata
TitleMOLECULAR IDENTIFICATION OF FRESHWATER PATHOGENIC OOMYCETES AND DETERMINATION OF NOVEL FACTORS THAT PROMOTE THEIR SPREADING
Title (english)MOLEKULARNA IDENTIFIKACIJA SLATKOVODNIH PATOGENIH OOMYCOTA I UTVRĐIVANJE NOVIH ČIMBENKA KOJI POTIČU NJIHOVO RASPROSTRANJIVANJE
AuthorDora Pavić
MentorAna Bielen (mentor)
Committee memberIvana Maguire (predsjednik povjerenstva)
Committee memberMarin Ježić (član povjerenstva)
GranterUniversity of Zagreb
Faculty of Science
(Department of Biology)
Zagreb
Defense date and country2023, Croatia
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Biology
Universal decimal classification
(UDC)		57 - Biological sciences. Physical anthropology. Biodiversity
Abstract
Aphanomyces astaci and Saprolegnia spp. are considered as the most destructive Oomycota pathogens of freshwater animals, both in natural habitats and in aquaculture, and this dissertation addressed several understudied aspects of their ecology. First, a highly sensitive S. parasiticaspecific ddPCR assay was developed, which allowed absolute quantification of the pathogen in environmental DNA samples. Further, the results obtained provided information on the microbiological and physico-chemical properties of natural waters that favour the sporulation and spread of these pathogens, such as environmentally relevant concentrations of humic acids. Finally, the role of aquaculture facilities as points of spreading of Saprolegnia spp. into downstream freshwater environments was demonstrated. Overall, the knowledge gained provides the basis for sensitive monitoring and predicting the various factors that could promote the spread of A. astaci and Saprolegnia spp. in the wild and aquaculture
Abstract (english)
Aphanomyces astaci i Saprolegnia spp. najznačajniji su oomicetni patogeni slatkovodnih životinja, kako u prirodnim staništima tako i u akvakulturi. U sklopu ove disertacije dobivena su nova saznanja o nekim aspektima njihove ekologije. Najprije je razvijen visoko osjetljiv i specifičan ddPCR-test za detekciju vrste S. parasitica, čime je po prvi puta omogućena apsolutna kvantifikacija ovog patogena u uzorcima okolišne DNA. Nadalje, utvrđena su mikrobiološka i fizikalno-kemijska svojstva prirodnih voda koja potiču sporulaciju i širenje oomicetnih patogena, kao što je prisutnost huminskih kiselina u okolišno relevantnim koncentracijama. Naposlijetku, demonstrirana je uloga uzgajališta pastrve u širenju patogena Saprolegnia spp. u nizvodna prirodna staništa. Zaključno, rezultati ove disertacije omogućit će praćenje oomicetnih patogena u prirodnim slatkovodnim staništima i akvakulturi, kao i predviđanje različitih čimbenika koji potiču njihovo rasprostranjivanje.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:217:876903
Promotion2023
Study programmeTitle: Biology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-05-04 13:09:29