Učinak visoke temperature uzgoja na bioaktivnost ekstrakata klijanaca brokule (Brassica oleracea convar. botrytis) tijekom modela ljudske probave in vitro

master's thesis

• Title (english): The effect of high temperature cultivation on the bioactivity of broccoli sprouts (Brassica oleracea convar. botrytis) extracts during in vitro model of human digestion
• Author: Silvija Gulin
• Mentor: Ivana Šola (mentor)
• Committee member: Ivana Šola (predsjednik povjerenstva)
• Committee member: Dario Hruševar (član povjerenstva)
• Committee member: Jasna Lajtner (član povjerenstva)
• Committee member: Duje Lisičić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2023-05-02, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Visoke temperature značajno mijenjaju sadržaj metabolita u biljkama, nutritivnu vrijednost te
biološke učinke ekstrakata biljaka. Cilj ovog rada bio je istražiti učinak visoke temperature
uzgoja (38°C dan, 33°C noć) na koncentraciju različitih skupina i pojedinačnih spojeva u
klijancima brokule (Brassica oleracea convar. botrytis) prije i tijekom in vitro simulirane
probave, kao i na njihov antioksidacijski i antiglikacijski, te potencijal inhibicije enzima αamilaze, α-glukozidaze i lipaze. Visoka temperatura uzgoja povećala je udio i biodostupnost
ukupnih fenolnih spojeva, flavonoida, flavonola, fenolnih i hidroksicimetnih kiselina u
klijancima brokule, no rezultati su varirali ovisno o fazama probave. Na pojedinačne fenolne
spojeve visoka temperatura uzgoja djelovala je značajnim sniženjem udjela vitamina C,
povećanjem ferulične kiseline te kvercetina, dok na udio kempferola nije utjecala.
Antioksidacijski potencijal ekstrakata klijanaca brokule porastao je prije probave mjeren
metodom ABTS, dok je metoda DPPH pokazala obrnuti učinak. Visoka temperatura uzgoja
smanjila je sposobnost ekstrakata da inhibiraju aktivnost enzima α-amilaze, povećala
sposobnost inhibicije aktivnostienzima α-glukozidaze i glikacije proteina, nonakon probave
rezultati su varirali ovisno o fazi probave i metodi mjerenja. Zaključujem da visoka
temperatura uzgoja znatno može pridonijeti kvaliteti prehrane klijancima kupusnjača, no
potrebne su detaljnije analize.

Abstract (english)

High temperatures significantly change metabolites content in plants, nutritional value and
biological effects of plant extracts. The aim of this study was to investigate the effect of high
growing temperature (38°C day, 33°C night) on the concentration of different groups and
individual compounds in broccoli seedlings (Brassica oleracea convar. botrytis) before and
during in vitro simulated digestion, as well as on their antioxidant, antiglycation and
inhibition potential of α-amylase, α-glucosidase and lipase enzymes. High cultivation
temperature increased the proportion and bioavailability of total phenolic compounds,
flavonoids, flavonols, phenolic and hydroxycinnamic acids in broccoli sprouts, but the results
varied depending on the stages of digestion. The high growing temperature affected individual
phenolic compounds by significantly reducing the content of vitamin C, increasing ferulic
acid and quercetin, while the content of kaempferol was not affected. The antioxidant
potential of broccoli sprout extracts increased before digestion measured by the ABTS
method, while the DPPH method showed the opposite effect. High cultivation temperature
reduced the ability of extracts to inhibit α-amylase enzyme activity, increased the ability to
inhibit α-glucosidase enzyme activity and protein glycation, but after digestion the results
varied depending on the digestion phase and measurement method. Therefore, high growing
temperature can significantly contribute to the quality of cabbage seedlings, but more detailed
analyzes are needed.

• Keywords: α–amilaza; α-glukozidaza; flavonoidi; RP-HPLC; spektrofotometrija; vitamin C
• Keywords (english): α –amylase; α-glucosidase; flavonoids; RP-HPLC; spectrophotometry; vitamin C
• Language: croatian
• URN:NBN: urn:nbn:hr:217:007743
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-05-02
• Created on: 2023-05-12 09:18:53