Razvoj metode tekućinske kromatografije ultravisoke djelotvornosti za analizu razgradnih produkata lijeka Entakapon

master's thesis

• Title (english): Development of ultra performance liquid chromatographic method for the analysis of Entacapone degradation products
• Author: Ani Knežević
• Mentor: Nives Galić (mentor)
• Committee member: Nives Galić (predsjednik povjerenstva)
• Committee member: Ines Primožič (član povjerenstva)
• Committee member: Biserka Prugovečki (član povjerenstva)
• Committee member: Iva Juranović Cindrić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2017-02-24, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

U ovom diplomskog radu razvijena je brza i učinkovita metoda tekućinske
kromatografije ultravisoke djelotvornosti (UHPLC) za analizu onečišćenja i razgradnih
produkata lijeka entakapona. Razvijenom metodom uspješno su odijeljeni pikovi entakapona,
onečišćenja i razgradnih produkata. Za određivanje optimalnih uvjeta kromatografskog
odjeljivanja metodom UHPLC, temperature i sastava pokretne faze, korišten je program
Drylab.
Vezani sustav LC-MS/MS također je korišten pri analizi... More razgradnih produkata. Na
temelju nastalih fragmenata pretpostavljene su strukture razgradnih produkata. Također su na
temelju MS/MS spektara određeni mogući načini fragmentacije razgradnih produkata lijeka
entakapona. Less

Abstract (english)

A quick and efficient method of high efficiency liquid chromatography (UHPLC)
for analysis of contaminants and degradation products of the drug entacapone was developed
through this diploma thesis. The developed methods had successfully separated peaks of
entacapone from contaminants and degradation products. Drylab software was used to
determine the optimal conditions of UHPLC chromatographic separation method, with
variables being temperature and composition of the mobile... More phase.
LC-MS/MS coupled system was used in the analysis of the degradation products.
Structure of the degradation products were assumed based on the resulting fragments.
Additionally, possible modes of entacapone degradation products fragmentation were
assessed by analyzing MS/MS spectra Less

• Keywords: entakapon; MS/MS; razgradni produkti; UHPLC metoda
• Keywords (english): entacapone; contaminants; degradation products; MS/MS; UHPLC method
• Language: croatian
• URN:NBN: urn:nbn:hr:217:975926
• Study programme: Title: Graduate university studies in chemistry; specializations in: Research programme; Course: Research programme; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-07-10 09:09:02