Učinak viška i manjka vode na biodostupnost bioaktivnih spojeva klijanaca brokule (Brassica oleracea convar. botrytis var. cymosa) te antioksidacijski kapacitet njihovih ekstrakata u modelu probave in vitro

master's thesis

• Title (english): The effect of excess and lack of water on the bioavailability of bioactive compounds in broccoli (Brassica oleracea convar. botrytis var. cymosa) seedlings and the antioxidant capacity of their extracts in an in vitro model of digestion
• Author: Ena Vilagoš
• Mentor: Ivana Šola (mentor)
• Committee member: Marin Ježić (predsjednik povjerenstva)
• Committee member: Domagoj Đikić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2023-09-19, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Učestalost ekstremnih klimatskih promjena poput poplava i suše povećava se svakodnevno,
zajedno sa opsegom štete koju uzrokuju. Klijanci kupusnjača popularna su namirnica zbog visokog
udjela fenolnih spojeva, vitamina, glukozinolata i vlakana, te mogućnosti lakog i jeftinog uzgoja,
što ih čini zanimljivima za istraživanja. Međutim, udjeli ovih spojeva u biljkama značajno ovise o
dostupnosti vode u tlu. Cilj ovog rada bio je usporediti učinak viška i manjka vode u tlu prilikom
uzgoja klijanaca brokule na biodostupnost fenolnih spojeva, proteina i vitamina te antioksidacijski
kapacitet etanolnih ekstrakata nakon in vitro simulacije ljudske probave u ustima, želucu i crijevu.
Metode spektrofotometrije i tekućinske kromatografije visoke moći razlučivanja (HPLC) pokazale
su značajan doprinos manjka vode većoj biodostupnosti folne kiseline i vitamina B12 nakon
probave u crijevima. S druge strane, isti uvjeti značajno smanjuju biodostupnost sinapinske
kiseline. Uvjeti viška vode pozitivno utječu na biodostupnost proteina i kempferola, a negativno
na ukupne fenole, flavonoide i vitamin biotin, kao i antioksidacijski kapacitet ekstrakata nakon
crijevne faze probave. Oba uvjeta vodnog stresa uzrokovala su smanjenje biodostupnosti ukupnih
hidroksicimetnih kiselina. Neproporcionalnost odnosa udjela i biodostupnosti nekih spojeva nakon
pojedinih faza probave sugerira utjecaj drugih faktora u biljnom materijalu, osim njihove
koncentracije, na biodostupnost.

Abstract (english)

Frequent extreme weather conditions caused by climate change, such as flooding and drought, have
been increasing their impact on the environment. Brassica sprouts are popular for their high content
of phenolics, vitamins, glucosinolates and fibers, which along with simple and inexpensive
production makes them an interesting subject of research. Considering the dependency of the
amount of these compounds on the availability of water in soil, the goal of this research was to
compare its effects during broccoli seedling growth on the bioavailability of phenolics, proteins
and vitamins, as well as the antioxidant capacity of their ethanol extracts after an in vitro simulation
of human digestion in the mouth, stomach and intestine. Methods of spectrophotometry and highperforming liquid chromatography (HPLC) showed that lack of water significantly increased the
bioavailability of folic acid and vitamin B12 after digestion in the intestinal phase, while the same
watering conditions significantly decreased the bioavailability of sinapic acid. Excess water had a
favorable effect on bioavailability of proteins and kaempferol, and negative on total phenolics,
flavonoids and vitamin biotin, along with the decrease of the extracts’ antioxidant capacity after
digestion in the intestinal phase. Both watering stress conditions caused lowering of total
hydroxycinnamic acid bioavailability. Disproportional values of amount and bioavailability of
some compounds after certain phases of digestion suggest the influence of other existing factors,
beside their concentration, on bioavailability.

• Keywords: bioraspoloživost; fenoli; klimatske promjene; mikrozelenje; proteini; vitamini; vodni stres
• Keywords (english): bioaccessibility; climate change; microgreens; phenolics; protein; vitamin; watering stress
• Language: croatian
• URN:NBN: urn:nbn:hr:217:649880
• Project: Number: IP-2020-02-7585; Title: Indirektni utjecaj globalnog zagrijavanja na fiziološke parametre sisavaca prehranom biljkama uzgojenim pri visokoj temperaturi; Title: Indirect Effect of Global Warming on Mammals Physiological Parameters via High Temperature-Stressed Plant Diet; Acronym: TEMPHYS; Leader: Ivana Šola; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: IP
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-09-19
• Created on: 2023-10-02 12:54:46