Ekspresija gena IL1B i CXCR4 u karcinomu pluća nemalih stanica bez mutacije u genu EGFR

master's thesis

• Title (english): The expression of ILIB and CXCR4 genes in non-small-cell lung cancer without mutations in EGFR gene
• Author: Matko Bazdan
• Mentor: Petra Korać (mentor)
• Mentor: Paula Gršković (komentor)
• Committee member: Petra Korać (predsjednik povjerenstva)
• Committee member: Biljana Balen (član povjerenstva)
• Committee member: Inga Urlić (član povjerenstva)
• Committee member: Maja Matulić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2023-09-25, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES

Abstract

Karcinom pluća najčešći je uzrok smrti povezanih s malignim bolestima. Dijeli se na karcinome
pluća malih i nemalih stanica. U ovom diplomskom radu istražit ću ekspresiju dvaju gena od
interesa, IL1B i CXCR4, koji su važni za imunosni odgovor organizma na razvoj karcinoma
pluća nemalih stanica koje nemaju mutaciju u genu za receptor epidermnog faktora rasta
(EGFR, od eng. epidermal growth factor receptor). Utvrđivanje mutacije u genu EGFR važno
je za liječenje karcinoma pluća nemalih stanica radi određivanja precizne terapije. IL1B kodira
interleukin-1 beta, a CXCR4 C-X-C kemokinski receptor 4. Oba imaju važnu, ali i nedovoljno
istraženu ulogu u imunosnom odgovoru na karcinom. Cilj ovog istraživanja jest utvrditi njihovu
razinu ekspresije u uzorcima karcinoma pluća nemalih stanica bez mutacije u genu EGFR u
odnosu na uzorke karcinoma pluća nemalih stanica s mutacijom u genu EGFR i tako omogućiti
bolje razumijevanje vrste imunosnog odgovora kod ovog entiteta. U tu svrhu izolirao sam RNA
gena od interesa koju sam preveo u cDNA kako bih mogao provesti qPCR. Razinu ekspresije
analizirao sam metodom kvantifikacije ddCt. Rezultati pokazuju povišenu ekspresiju
istraživanih gena u karcinomu pluća nemalih stanica bez mutacije gena EGFR u odnosu na isti
tip karcinoma koji ima mutaciju u tome genu.

Abstract (english)

Lung cancer is the most common malignancy-related cause of death. It can be divided into two
major groups: non-small cell lung cancer (NSCLC) and small cell lung cancer (SCLC). In this
thesis, I will analyze the expression of two genes, IL1B and CXCR4 which are important factors
in the immune response in NSCLC without mutations in EGFR gene. Mutations in EGFR gene
are important because based on their presence in the cancer targeted therapy can be applied.
IL1B encodes the protein interleukin-1 beta, and CXCR4 encodes chemokine C-X-C receptor
type 4. Both of these proteins have a significant, but still inadequately explored role in the
immune response against cancer. The aim of this research is to determine the expression of
these two genes in NSCLC without mutations in EGFR gene relative to samples that have the
mutation to further understand the immune response of this cancer subtype. I have isolated the
RNA of the two genes and translated it into cDNA for multiplying via qPCR. I determined the
expression levels using the ddCt method. Expression of the studied genes was elevated in
NSCLC without mutations in EGFR gene relative to the same cancer type that has the mutated
gene.

• Keywords: receptor; citokin; upala
• Keywords (english): receptor; cytokine; inflammation
• Language: croatian
• URN:NBN: urn:nbn:hr:217:375630
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-09-25
• Created on: 2023-10-17 11:42:17