N-GLYCOSYLATION OF IMMUNOGLOBULIN G AND TOTAL PLASMA PROTEINS IN CORONARY ARTERY DISEASE

doctoral thesis

• Title (croatian): N-GLIKOZILACIJA IMUNOGLOBULINA G I UKUPNIH PROTEINA PLAZME U KORONARNOJ BOLESTI SRCA
• Author: BARBARA RADOVANI
• Mentor: Ivan Gudelj (mentor)
• Committee member: Olga Gornik Kljaić (predsjednik povjerenstva)
• Committee member: Petra Korać (član povjerenstva)
• Committee member: Toma Keser (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2023-10-19, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 577 - Biochemistry. Molecular biology. Biophysics

Abstract

Coronary artery disease (CAD) is the most common cardiovascular disease (CVD), resulting
from chronic inflammation of the coronary arteries due to the formation of atherosclerotic
plaques, and its presence is a significant marker of adverse cardiovascular (CV) events.
Previous research has linked N-glycosylation, a highly regulated posttranslational
modification, to the development of atherosclerotic plaques. Therefore, the aim of this work
was to determine whether N-glycosylation of total plasma proteins and, in particular,
immunoglobulin G (IgG) is associated with CAD. Sex-stratified analysis of N-glycosylation
of total plasma proteins and IgG revealed significant changes associated with CAD, with
different patterns observed in men and women. Highly branched sialylated plasma N-glycan
structures with terminal fucose were positively associated, whereas biantennary sialylated IgG
N-glycan structures were negatively associated with CAD. Overall, protein N-glycosylation
emerges as a significant factor in CAD, with glycan-based biomarkers showing promise for
predicting cardiovascular health.

Abstract (croatian)

Koronarna bolest srca (KBS) najčešća je kardiovaskularna bolest (KVB), koja nastaje kao
posljedica kronične upale koronarnih arterija uslijed stvaranja aterosklerotskih plakova, a
njezina je prisutnost značajan biljeg štetnih kardiovaskularnih (KV) događaja. Prethodna su
istraživanja povezala N-glikozilaciju, visoko reguliranu posttranslacijsku modifikaciju, s
razvojem aterosklerotskih plakova. Stoga je cilj ovog doktorskog rada bio utvrditi je li Nglikom ukupnih proteina plazme, a posebice imunoglobulina G (IgG), povezan s KBS-om.
Spolno stratificirana analiza N-glikozilacije ukupnih proteina plazme i IgG otkrila je značajne
promjene povezane s KBS-om, s različitim obrascima uočenim u muškaraca i žena. Visoko
razgranate sijalizirane N-glikanske strukture proteina plazme s terminalnom fukozom bile su
pozitivno povezane, dok su biantenarne sijalizirane IgG N-glikanske strukture bile negativno
povezane s KBS-om. Sve u svemu, N-glikozilacija proteina pojavljuje se kao značajan
čimbenik u KBS-u, te biljezi temeljeni na glikanima predstavljaju potencijal za predviđanje
kardiovaskularnog zdravlja.

• Keywords: glycosylation; immunoglobulin G; plasma proteins; coronary artery disease; inflammation; biomarker
• Keywords (croatian): glikozilacija; imunoglobulin G; proteini plazme; koronarna bolest srca; upala; biomarker
• Language: english
• URN:NBN: urn:nbn:hr:217:709810
• Promotion: 2023
• Project: Number: UIP-2019-04-5692; Title: Glikozilacija u kardiovaskularnim bolestima; Title: Glycosylation in Cardiovascular diseases; Acronym: GLYCARD; Leader: Ivan Gudelj; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: UIP
• Study programme: Title: Doctoral study; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti u području prirodnih znanosti (doktor/doktorica znanosti u području prirodnih znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-10-25 08:15:38