Genetički dokazi hibridizacije između planinskog (Teucrium montanum L.) i pustenastog dubačca (Teucrium polium L.)

master's thesis

• Title (english): Genetic evidences of hybridization between mountain (Teucrium montanum L.) and felty germander (Teucrium polium L.)
• Author: Lea Juzbašić
• Mentor: Zlatko Liber (mentor)
• Committee member: Zlatko Liber (predsjednik povjerenstva)
• Committee member: Mirela Sertić Perić (član povjerenstva)
• Committee member: Draginja Mrvoš-Sermek (član povjerenstva)
• Committee member: Biserka Prugovečki (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2023-09-18, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Ovim diplomskim radom se po prvi put molekularnim pristupom istražila spontana interspecijska
hibridizacija između vrsta Teucrium montanum L. i T. polium L.. Primijenjeno je 12 novorazvijenih
mikrosatelitnih biljega zajedničkih za obje vrste. Istražen je polimorfizam mikrosatelitnih lokusa 70
jedinki roditeljskih vrsta i njihovih hibrida skupljenih na simpatričnom lokalitetu Vojnić Sinjski. Novi
mikrosatelitni biljezi uspješno su razlučili obje roditeljske vrste kao i njihove hibride. Uz nedostatak F1
i F2 hibrida utvrđena je dominacija povratnih hibrida. Dominacija povratnog križanja u odnosu na
međusobno križanje hibrida ukazuje na introgresiju kao primarni ishod, a ne na specijaciju hibrida.
Premda T. montanum i T. polium zauzimaju jako široke ekološke niše, ekološki uvjeti na lokalitetu
Vojnić Sinjski mnogo su bliži ekološkom optimumu nešto termofilnije vrste T. polium pa ne čudi puno
češće povratno križanje baš s ovom vrstom. Buduća primjena novorazvijenih mikrosatelitnih biljega na
cjelokupnim arealima omogućit će rasvjetljavanje zamršene taksonomije, filogeografije i hibridizacije
ove dvije usko srodne vrste unutar sekcije Polium roda Teucrium.

Abstract (english)

In this thesis, spontaneous interspecific hybridisation between the species Teucrium montanum L. and
T. polium L. was studied for the first time using a molecular approach. Twelve newly developed
microsatellite markers common to both species were applied. The microsatellite loci polymorphism of
70 individuals of the two parental species and their hybrids collected at the sympatric locality Vojnić
Sinjski was studied. The new microsatellite markers successfully distinguished both parent species as
well as their hybrids. Besides the absence of F1 and F2 hybrids, the dominance of backcross hybrids
was observed. The dominance of backcrosses over hybrid intercrossing suggests that introgression is
the primary outcome rather than hybrid speciation. Although T. montanum and T. polium occupy very
broad ecological niches, the ecological conditions at the Vojnić Sinjski site are much closer to the
ecological optimum of the more thermophilic T. polium, so it is not surprising that backcrosses with
this species are much more common. The future application of the newly developed microsatellite
markers to the entire distribution ranges will make it possible to clarify the complicated taxonony,
phylogeography and hybridisation of these two closely related species within the section Polium of the
genus Teucrium.

• Keywords: mikrosateliti; hibridi; introgresija
• Keywords (english): microsatellite; hybrids; introgression
• Language: croatian
• URN:NBN: urn:nbn:hr:217:670745
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-09-18
• Created on: 2023-10-25 10:18:05