Obilježja perineuronskih mreža u prefrontalnoj kori mozga čovjeka

master's thesis

• Title (english): Characteristics of perineuronal nets in the prefrontal cortex of the human brain
• Author: Ivona Kirchbaum
• Mentor: Ivan Banovac (mentor)
• Mentor: Domagoj Đikić (komentor)
• Committee member: Domagoj Đikić (predsjednik povjerenstva)
• Committee member: Petra Peharec Štefanić (član povjerenstva)
• Committee member: Nada Oršolić (član povjerenstva)
• Committee member: Dubravka Hranilović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2023, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Prefrontalna moždana kora (PFC) nalazi se na prednjem dijelu čeonog režnja mozga i proteže se
na njegovoj medijalnoj, lateralnoj i orbitalnoj površini. PFC može se podijeliti na dvije regije s
različitim funkcijama: lateralni PFC (LPFC) (dorzolateralni i ventrolateralni PFC) i
ventromedijalni PFC (vmPFC). Osim na regije, PFC možemo podijeliti na temelju razlika u
citoarhitektonici u Brodmannova polja. Izvanstanični matriks PFC-a sadrži složenu mješavinu
proteoglikana, tenascina, fibronektina i hijaluronske kiseline koja može okruživati stanična tijela i
nastavke neurona, tvoreći dobro organiziranu rešetkastu strukturu koja se naziva perineuronskom
mrežom (PNN). PNN-ovi su uključeni u neuroprotekciju, sinaptičku stabilizaciju, ionsko
puferiranje te razvoj i plastičnost neurona. Hipoteza ovog diplomskog rada jest da postoje razlike
u udjelu, raspodjeli po slojevima i obilježjima perineuronskih mreža između ventralnih i dorzalnih
dijelova PFC-a čovjeka. Hipotezu sam ispitala određivanjem udjela PNN-ova u ukupnom broju
neurona, raspodjele PNN-ova po slojevima moždane kore te stupnja kolokalizacije PNN-ova i
biljega interneurona u ventralnim i dorzalnim dijelovima moždane kore čovjeka. Rezultati
imunohistokemijske analize pokazuju da ne postoji značajna razlika u raspodjeli po slojevima i
udjelu PNN-ova u ukupnom broju neurona između BA9 i BA14. S druge strane, uočene su
određene razlike u raspodjeli PNN-ova uokolo pojedinih subpopulacija živčanih stanica između
BA9 i BA14r.

Abstract (english)

The prefrontal cortex (PFC) is located in the frontal lobe of the brain and extends over its medial,
lateral, and orbital surfaces. The PFC can be divided into two regions with different functions: the
lateral PFC (LPFC) (dorsolateral and ventrolateral PFC) and ventromedial PFC (vmPFC).
Furthermore, the PFC can be divided based on differences in cytoarchitectonics into Brodmann
areas. The extracellular matrix of the PFC contains a complex mixture of proteoglycans, tenascins,
fibronectin, and hyaluronic acid that can surround the cell bodies and processes of neurons, forming
a well-organized structure called perineuronal nets (PNNs). PNNs are involved in neuroprotection,
synaptic stabilization, ion buffering, and neuronal development and plasticity. The hypothesis of
this thesis is that there are differences in the proportion, laminar distribution, and characteristics of
perineuronal nets between the ventral and dorsal parts of the human PFC. I tested the hypothesis
by determining the proportion of PNNs in the total number of neurons, the distribution of PNNs
by layers of the cerebral cortex, and the degree of colocalization of PNNs and interneuron markers
in the ventral and dorsal parts of the human cerebral cortex. The results of the
immunohistochemical analysis revealed that there is no significant difference in the distribution by
layers and the proportion of PNNs in the total number of neurons between BA9 and BA14. On the
other hand, certain differences were observed in the distribution of PNNs around specific
subpopulations of neurons between BA9 and BA14r

• Keywords: izvanstanični matriks; živčane stanice; interneuroni; parvalbumin
• Keywords (english): extracellular matrix; nerve cells; interneurons; parvalbumi
• Language: croatian
• URN:NBN: urn:nbn:hr:217:636036
• Project: Number: IP-2019-04-3182; Title: Moždani izvanstanični matriks u razvitku i perinatalnoj hipoksiji; Acronym: BRAINECM; Leader: Nataša Jovanov Milošević; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: IP
• Project: Number: KK.01.1.1.01.0007; Title: Eksperimentalna i klinička istraživanja hipoksijsko-ishemijskog oštećenja mozga u perinatalnoj i odrasloj dobi; Title: Eksperimentalna i klinička istraživanja hipoksijsko-ishemijskog oštećenja mozga u perinatalnoj i odrasloj dobi; Leader: Ivica Kostović; Jurisdiction: Croatia; Funder: EK
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2023-10-25 10:38:34