Utjecaj primjene alfa-cipermetrina u trudnoći na obrasce metilacije DNA u neonatalnom i pubertetnom jajniku štakora

master's thesis

• Title (english): The impact of alfa-cypermethrin administration in the gestational period on DNA methylation patterns in neonatal and pubertal rat ovary
• Author: Lorena Beretin
• Mentor: Ana Katušić Bojanac (mentor)
• Mentor: Inga Urlić (komentor)
• Committee member: Inga Urlić (predsjednik povjerenstva)
• Committee member: Petra Korać (član povjerenstva)
• Committee member: Maja Matulić (član povjerenstva)
• Committee member: Tomislav Ivanković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2023-11-09, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Metilacija DNA prva je otkivena te najviše istraživana epigenetička oznaka. Ova oznaka
nalazi se unutar same molekule DNA, a posebnu ulogu ima tijekom uspostavljanja
metilacijskog statusa utisnutih gena čime se postiže spolno-specifična ekspresija gena.
Pravilna uspostava metilacije utisnutih gena preduvjet je za normalan razvoj te reproduktivnu
sposobnost potomaka. Sintetski piretroidni insekticid α-cipermetrin djeluje kao endokrini
disruptor uzrokujući promjene u epigenetičkim mehanizmima čime dolazi do promjena u
regulaciji ekspresije gena. U ovom radu istražen je utjecaj prenatalne izloženosti α-
cipermetrinu na promjene u obrascu metilacije DNA u neonatalnom i u pubertetnom jajniku
štakora. Metodom pirosekvenciranja analizirani su retrotranspozonski sljedovi LINE-1 koji
služe za procjenu globalne metilacije, te diferencijalno metilirane regije utisnutih gena Igf2r i
Mest. Dobiveni rezultati nisu rezultirali statistički značajnim razlikama između kontrolnih i
eksperimentalnih skupina. Ovaj rad prvo je istraživanje kojim se analizirao utjecaj prenatalne
izloženosti α-cipermetrinu na obrasce metilacije DNA u neonatalnom i pubertetnom jajniku
štakora.

Abstract (english)

DNA methylation is the first identified and most researched epigenetic mark. This mark is
located inside the DNA molecule itself, and it plays a special role during the establishment of
the methylation status of imprinted genes, which achieve sex-specific gene expression. Proper
establishment of methylation of imprinted genes is a prerequisite for normal development and
reproductive capacity of offspring. The synthetic pyrethroid insecticide α-cypermethrin acts
as an endocrine disruptor, causing changes in epigenetic mechanisms, which lead to changes
in the regulation of gene expression. In this paper, the impact of α-cypermethrin
administration in the gestational period on the DNA methylation patterns in the neonatal and
pubertal rat ovary was investigated. Using the pyrosequencing method, LINE-1
retrotransposon sequences were analyzed, which were used to assess global methylation, as
well as differentially methylated regions of the imprinted genes Igf2r and Mest. There were
no statistically significant differences between the control and experimental groups. This
work is the first study that analyzed the influence of prenatal exposure to α-cypermethrin on
DNA methylation patterns in neonatal and pubertal rat ovaries.

• Keywords: endokrini disruptori; genomski utisak; pirosekvenciranje; reproduktivni razvoj
• Keywords (english): endocrine disruptors; genomic imprinting; pyrosequencing; reproductive development
• Language: croatian
• URN:NBN: urn:nbn:hr:217:127971
• Study programme: Title: Molecular Biology-University graduate programme; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra molekularne biologije (sveučilišni/a magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-11-09
• Created on: 2023-11-22 13:37:43