Nanomorfološka karakterizacija stanica mikroalga i rekonstruiranih membranskih vezikula

master's thesis

• Title (english): Nanomorphological characterization of microalgal cells and reconstructed membrane vesicles
• Author: Karlo Ojdanić
• Mentor: Nadica Ivošević DeNardis (mentor)
• Committee member: Iva Juranović Cindrić (predsjednik povjerenstva)
• Committee member: Ines Primožič (član povjerenstva)
• Committee member: Mirta Rubčić (član povjerenstva)
• Committee member: Tomislav Jednačak (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2023-09-22, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

U ovom je diplomskom radu provedena nanomorfološka karakterizacija triju staničnih vrsta mikroalga i rekonstruiranih membranskih vezikula. Rekonstruirane membranske vezikule pripremljene su hipoosmotskim šokom stanica različite kompleksnosti stanične barijere. Stanice mikroalga i rekonstruirane vezikule karakterizirane su u smislu veličine, oblika i strukture primjenom mikroskopije atomskih sila. Oslikane stanice bile su pojedinačne, mikrometarske veličine, elipsoidnog ili sfernog oblika uz karakteristične flagele i biokalcitne strukture na površini. Oslikane vezikule bile su mikrometarske veličine, pojedinačne, sfernog ili ovalnog oblika gotovo prazne ili pune. Strukturne karakteristike rekonstruiranih vezikula i ljušturica ovise o kompleksnosti stanične barijere. Rezultati su pokazali da se vezikule mogu reverzibilno rekonstruirati hidratizacijom liofiliziranog vezikularnog materijala što može osigurati produljeno čuvanje materijala. Rezultati ovog rada mogu doprinijeti daljnjem razvoju biotehnološkog potencijala mikroalga iz vodenog ekosustava za pripremu rekonstruiranih membranskih vezikula kao potencijalnog transportnog sustava aktivnih komponenti.

Abstract (english)

In this thesis, nanomorphological characterization of three microalgal cell species and reconstructed membrane vesicles was carried out. Reconstructed membrane vesicles were prepared by hypoosmotic shock of cells with different cell barrier complexity. Microalgal cells and reconstructed vesicles were characterized in terms of size, shape and structure using atomic force microscopy. The imaged cells were single, micrometer in size, ellipsoidal or spherical in shape with characteristic flagella and biocalcite structures on the surface. The imaged vesicles were micrometer-sized, single, spherical or oval in shape, almost empty or full. The structural features of the reconstructed vesicles and theca depend on the complexity of the cellular barrier. The results showed that vesicles can be reversibly reconstructed by hydration of lyophilized vesicular material, which can ensure prolonged storage of the material. The results of this work can contribute to the further development of the biotechnological potential of microalgae from the aquatic ecosystem for the preparation of reconstructed membrane vesicles as a potential transport system of active components.

• Keywords: Dunaliella tertiolecta; mikroskopija atomskih sila; nanomorfologija rekonstruirane membranske vezikule; Rhodomonas maculata; Tetraselmis suecica
• Keywords (english): atomic force microscopy; Dunaliella tertiolecta; nanomorphology; reconstructed membrane vesicles; Rhodomonas maculata; Tetraselmis suecica
• Language: croatian
• URN:NBN: urn:nbn:hr:217:218530
• Study programme: Title: Graduate university studies in chemistry; specializations in: Research programme; Course: Research programme; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-09-22
• Created on: 2023-11-22 14:20:00