Kvantitativno određivanje neoniktinoida i njihovih metabolita u pčelama i pčelinjim proizvodima vezanim sustavom tekućinska kromatografija - tandemna spektrometrija masa

doctoral thesis

• Title (english): Quantitative determination of neonicotinoides and its metabolites in honeybees and honeybee products using liquid chromatography-tandem mass spectrometry
• Author: Damir Pavliček
• Mentor: Ivana Tlak Gajger (mentor)
• Mentor: Nina Bilandžić (mentor)
• Committee member: Nives Galić (predsjednik povjerenstva)
• Committee member: Darija Lemić (član povjerenstva)
• Committee member: Andreja Prevendar Crnić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2023-12-01, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry
• Universal decimal classification (UDC): 54 - Chemistry. Crystallography. Mineralogy

Abstract

Razvijena je metoda za kvantitativno određivanje neonikotinoida i njihovih metabolita u
uzorcima pčela i pčelinjim proizvodima vezanim sustavom tekućinska kromatografija –
tandemna spektrometrija masa. Predloženi analitički postupci validirani su u skladu sa
smjernicama SANTE/12682/2019 ispitivanjem radnih značajki metode u svim ciljanim
matricama. Preciznost, izražena kao relativna standardna devijacija, bila je < 20 %, dok je
točnost, izražena kao analitički povrat bila u rasponu od... More 70 % do 120 %, za većinu
neonikotinoida i njihovih metabolita. U okviru validacije metoda procijenjena mjerna
nesigurnost iznosila je < 50 % za sve analite. Predloženom metodom analizirano je ukupno
664 uzoraka pčela i pčelinjih proizvoda uzorkovanih tijekom 2019. i 2020. godine u svim
regijama Republike Hrvatske. Ostatci neonikotinoida i njihovih metabolita detektirani su u
17,6 % uzoraka, a tiakloprid je bio najčešće prisutan analit u obje godine istraživanja. Less

Abstract (english)

A method for the quantitative determination of neonicotinoids and their metabolites in
honeybee and bee product samples using the liquid chromatography - tandem mass
spectrometry system was developed. The proposed analytical procedures were validated in
accordance with the SANTE/12682/2019 guidelines by evaluating the performance
characteristics of the method in all target matrices. Precision, expressed as relative standard
deviation, was < 20 %, while accuracy, expressed as... More analytical recovery, ranged from 70 %
to 120 %, for most neonicotinoids and their metabolites. As part of method validation, the
estimated measurement uncertainty was < 50 % for all analytes. A total of 664 samples of
honeybees and bee products, sampled during 2019 and 2020 in all regions of the Republic of
Croatia, were analysed using the proposed method. Residues of neonicotinoids and their
metabolites were detected in 17.6 % of samples, and thiacloprid was the most frequently
present analyte in both years of the study. Less

• Keywords: LC-MS/MS; neonikotinoidi; pčela; pčelinji proizvodi; QuEChERS; validacija
• Keywords (english): bee products; honeybee; LC-MS/MS; neonicotinoids; QuEChERS; validation
• Language: croatian
• URN:NBN: urn:nbn:hr:217:377856
• Promotion: 2023
• Study programme: Title: Doctoral study; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica u području prirodnih znanosti (doktor/doktorica u području prirodnih znanosti)
• Type of resource: Text
• Extent: LVI, 193 str. ; 30 cm
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-12-01
• Created on: 2023-12-19 14:59:36