Molekularno-filogenetska analiza palearktičkih vrsta roda Limnius (Coleoptera: Elmidae)

master's thesis

• Title (english): Molecular-phylogenetic analysis of Palaearctic species of the genus Limnius (Coleoptera: Elmidae)
• Author: Karmen Radovčić
• Mentor: Branka Bruvo Mađarić (mentor)
• Mentor: Anamaria Štambuk (komentor)
• Committee member: Anamaria Štambuk (predsjednik povjerenstva)
• Committee member: Ana Galov (član povjerenstva)
• Committee member: Vlatka Mičetić Stanković (član povjerenstva)
• Committee member: Marija Ivković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2023-12-19, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Rod Limnius Illiger, 1802 pripada porodici vodenih kornjaša Elmidae (hrv. slapoljubi). Do sada je
unutar roda Limnius opisano 14 vrsta rasprostranjenih u palearktičkom području, a preliminarne
molekularne analize ukazuju na složene sistematske odnose unutar ovoga roda koji zahtijevaju
dodatna istraživanja. U sklopu ovog rada odredila sam filogenetske odnose između vrsta unutar
roda Limnius za uzorke prikupljene u zapadno-palearktičkoj regiji upotrebom mitohondrijskog
gena COI te nuklearnih gena ArgK, H3, CAD i ITS1. Za pojedinačne markere kao i za kombinacije
setova markera su konstruirana filogenetska stabla raznim filogenetskim metodama. Rezultati su
uglavnom usporedivi između upotrijebljenih setova podataka, no najbolju rezoluciju daje
kombinirani set svih molekularnih markera. Dobiveni rezultati potvrđuju prethodne filogenetske
hipoteze temeljene na morfologiji o bliskim odnosima vrsta L. volckmari / L. intermedius, L.
muelleri / L. satanus, L. opacus / L. subopacus i L. perrisi / L. colchicus, no odnosi između
pojedinih skupina vrsta nisu sa sigurnošću potvrđeni. Također, rezultati ukazuju na postojanje
određenih kompleksa vrsta kao i neopisanih (kriptičnih) vrsta unutar ovoga roda. Ovo istraživanje
daje važan doprinos poznavanju sistematike unutar roda Limnius i predstavlja osnovu za buduća
sveobuhvatna taksonomska i filogenetska istraživanja upotrebom integrativnog pristupa.

Abstract (english)

The genus Limnius Illiger, 1802 belongs to the family of aquatic beetles Elmidae (riffle beetles).
So far, 14 species distributed in the Palearctic region have been described within the genus, and
preliminary molecular analyzes indicate complex systematic relationships that require additional
research. In this work I determined the phylogenetic relationships between species within the genus
Limnius for samples collected in the Western Palearctic region using the mitochondrial gene COI
and the nuclear genes ArgK, H3, CAD and ITS1. Phylogenetic trees were constructed using various
phylogenetic methods for individual markers as well as for combinations of marker sets. The results
are mostly comparable between the data sets used, but the best resolution is provided using the
combined set of all molecular markers. The obtained results confirm previous phylogenetic
hypotheses based on morphology about the close relationships of the species L. volckmari / L.
intermedius, L. muelleri / L. satanus, L. opacus / L. subopacus and L. perrisi / L. colchicus, but the
relationships between the species groups have not been confirmed with certainty. Also, the results
indicate the existence of certain species complexes as well as undescribed (cryptic) species within
this genus. This research makes an important contribution to the knowledge of systematics within
the genus Limnius and represents the basis for future comprehensive taxonomic and phylogenetic
studies using an integrative approach.

• Keywords: slapoljubi; molekularna filogenetika; filogenetsko stablo; integrativna taksonomija; kompleksi vrsta; kriptične vrste
• Keywords (english): riffle beetles; molecular phylogenetics; phylogenetic tree; integrative taxonomy; species complex; cryptic species
• Language: croatian
• URN:NBN: urn:nbn:hr:217:081693
• Study programme: Title: Experimental Biology-University graduate programme; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra eksperimentalne biologije (sveučilišni/a magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-12-19
• Created on: 2024-01-11 11:19:17