Identifikacija i delimitacija vrsta roda Elmis (Coleoptera: Emidae) DNA barkodiranjem

master's thesis

• Title (english): Identification and delimitation of species within the genus Elmis (Coleoptera: Elmidae) using DNA barcoding
• Author: Ana Mrše
• Mentor: Branka Bruvo Mađarić (mentor)
• Mentor: Anamaria Štambuk (komentor)
• Committee member: Anamaria Štambuk (predsjednik povjerenstva)
• Committee member: Ana Galov (član povjerenstva)
• Committee member: Vlatka Mičetić Stanković (član povjerenstva)
• Committee member: Marija Ivković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2023-12-19, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Rod Elmis Latreille, 1802 pripada porodici vodenih kornjaša slapoljuba (Elmidae, Coleoptera). Slapoljubi
su osjetljivi na okolišne promjene te se koriste kao bioindikatori kvalitete tekućica, no njihova znatna
morfološka varijabilnost često otežava standardnu identifikaciju vrsta. DNA barkodiranje metoda je koja
upotrebom podjedinice I mitohondrijalnog gena za citokrom oksidazu (COI) omogućava brzu identifikaciju
vrsta i detekciju kriptičnih vrsta. Glavni cilj ovog diplomskog rada je identifikacija i delimitacija vrsta roda
Elmis DNA barkodiranjem, za uzorke prikupljene u zapadno-palearktičkom području. Uzorcima je
izdvojena ukupna genomska DNA, umnožen je i sekvenciran COI gen, konstruirana su filogenetska stabla,
provedene su metode molekularne identifikacije i delimitacije vrsta, te procjena vremena odvajanja na
temelju molekularnog sata. Za većinu uzoraka je potvrđena morfološka identifikacija, a nekoliko uzoraka
vjerojatno pripada još neopisanim vrstama. Delimitirano je ukupno 14 odvojenih taksonomskih jedinica
(MOTU-a), od kojih šest predstavlja nove MOTU-e za bazu BOLD. Vremenski kalibrirano stablo pokazalo
je da se većina sestrinskih vrsta odvojila u periodu od srednjeg pliocena do ranog pleistocena, a grupe vrsta
u miocenu. Rezultati ukazuju na postojanje još neopisanih vrsta kao i mogućih kompleksa vrsta, te upućuju
na potrebu detaljne taksonomske revizije roda Elmis.

Abstract (english)

The genus Elmis Latreille, 1802 belongs to the family of riffle beetles (Elmidae, Coleoptera). Elmidae are
sensitive to environmental changes and are used as bioindicators of water quality, but their considerable
morphological variability often makes standard species identification difficult. DNA barcoding is a method
which enables rapid identification of species and detection of cryptic species via analysis of the sequence
of mitochondrial gene for cytochrome oxidase, subunit I (COI). The main goal of this thesis is the
identification and delimitation of species of the genus Elmis by DNA barcoding, for samples collected in
the Western Palearctic area. Total genomic DNA was extracted from the samples, the COI gene was
amplified and sequenced, phylogenetic trees were constructed, methods of molecular identification and
species delimitation were performed, and separation time was estimated based on the molecular clock.
Morphological identification has been confirmed for most samples, while a few samples probably belong
to yet undescribed species. A total of 14 separate taxonomic units (MOTUs) were delimited, six of which
represent new MOTUs for the BOLD database. The time-calibrated tree showed that most of the sister
species separated in the period from the middle Pliocene to the early Pleistocene, while species groups
separated in the Miocene. The results indicate the existence of as yet undescribed species as well as possible
species complexes, and indicate the need for a detailed taxonomic revision of the genus Elmis.

• Keywords: slapoljubi; integrativna taksonomija; COI; molekularna delimitacija vrsta; molekularni sat; filogenetsko stablo
• Keywords (english): riffle beetles; integrative taxonomy; COI; molecular species delimitation; molecular clock; phylogenetic tree
• Language: croatian
• URN:NBN: urn:nbn:hr:217:085270
• Study programme: Title: Experimental Biology-University graduate programme; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra eksperimentalne biologije (sveučilišni/a magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-12-19
• Created on: 2024-01-15 13:16:25