PHYLOGENETIC RELATIONSHIP WITHIN GENUS Phoxinus (TELEOSTEI: CYPRINIDAE) IN THE WESTERN BALKANS

doctoral thesis

• Title (croatian): FILOGENETSKI ODNOSI UNUTAR RODA Phoxinus (TELEOSTEI: CYPRINIDAE) NA PODRUČJU ZAPADNOG BALKANA
• Author: Matej Vucić
• Mentor: Göran Klobučar (mentor)
• Mentor: Frédéric Grandjean https://orcid.org/0000-0002-8494-0985 (komentor)
• Committee member: Ivana Maguire (predsjednik povjerenstva)
• Committee member: Damjan Franjević (član povjerenstva)
• Committee member: Marina Piria (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2024-01-26, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 57 - Biological sciences. Physical anthropology. Biodiversity

Abstract

Minnows (genus Phoxinus) are small, cyprinid species of fish, widely distributed
throughout Europe and Asia, including in Croatia and neighboring countries. Currently, the
genus Phoxinus has as many as 23 different mitochondrial genetic lineages, thirteen
described species, while three species have been named, but their status has not yet been
confirmed. Until a few years ago, it was thought that two species were widespread in the
Western Balkans: Eurasian minnow (Phoxinus phoxinus) and the Adriatic minnow (P.
lumaireul). The first investigations showed the absence of the P. phoxinus species in this
area and the exceptional diversity of the P. lumaireul species with the existence of new
genetic lines and undescribed species. The aim of this doctoral investigation is to provide a
more detailed and clearer insight into the phylogenetic and phylogeographic as well as interand
intra-species relationships within the genus Phoxinus by a combination of molecular
phylogenetics and population genetics methods. The research showed a wide distribution of
the species P. lumaireul and a great genetic diversity in the Western Balkans, with the
existence of four genetic subclades in the area of Croatia and Slovenia. Two new species of
the Phoxinus genus were discovered in Croatia, one of which is new to science. By
combining the methods of molecular phylogenetics with morphology and meristics, this
species was described as Phoxinus krkae. For the needs of more detailed research on
population genetics within the genus, but especially within the P. lumaireul s.l. complex, a
new set of microsatellite markers was developed, which will provide detailed insights into
mutual population relationships, hybridization events and natural adn human-mediated
introgressions.

Abstract (croatian)

Pijori (rod Phoxinus) su male, ciprinidne vrste riba, široko rasprostranjenje na
području cijele Europe i Azije, pa tako i na području Hrvatske i susjednih zemalja. Trenutno
rod Phoxinus broji čak 23 različite mitohondijske genetičke linije, trinaest opisanih vrsta,
dok je trima vrstama dodijeljeno ime, ali njihov status još nije potvrđen. Do prije nekoliko
godina se smatralo da su na području Zapadnog Balkana rasprostranjene dvije vrste: pijor
(Phoxinus phoxinus) i tzv. jadranski pijor (P. lumaireul). Prva istaživanja su pokazala
nepostojanje vrste P. phoxinus na ovom području te iznimnu raznolikost vrste P. lumaireul
uz postojanje novih genetičkih linija i neopisanih vrsta. Cilj ovog rada je pružiti detaljniji i
jasniji uvid u filogenetske i filogeografske te inter- i intra-specijske odnose unutar Phoxinus
roda kombinacijom raznih molekularne filogenije i populacijske genetike. Istraživanje je
pokazalo široku rasprostranjenost vrste P. lumaireul te veliku genetsku raznolikost na
području Zapadnog Balkana, uz postojanje četiri genetičke podlinije na području Hrvatske i
Slovenije. Otkrivene su i dvije nove vrste roda Phoxinus na području Hrvatske, od kojih
jedna nova za znanost. Kombinacijom metoda molekularne filogenetike te morfologije i
meristike, ova vrsta je opisana kao Phoxinus krkae. Za potrebe detaljnijih istraživanja
populacijske genetike unutar roda, ali posebice unutar kompleksa P. lumaireul s.l., razvijen
je novi set mikrosatelitnih markera, koji će dati detaljne uvide u međusobne populacijske
odnose, hibridizacije te prirodne i ljudski uzrokovane introgresije.

• Keywords: Phoxinus; phylogenetics; relationships; genetic diversity; Western Balkans
• Keywords (croatian): Phoxinus; filogenetika; odnosi; genetička raznolikost; zapadni Balkan
• Language: english
• URN:NBN: urn:nbn:hr:217:568806
• Promotion: 2024
• Study programme: Title: Doctoral study; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti u području prirodnih znanosti (doktor/doktorica znanosti u području prirodnih znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-01-26
• Created on: 2024-02-26 13:08:19