N GLIKOZILACIJA SERUMSKIH PROTEINA I IMUNOGLOBULINA A U ŠEĆERNOJ BOLESTI TIPA 1

doctoral thesis

• Title (english): N-GLYCOSYLATION OF TOTAL SERUM PROTEINS AND IMMUNOGLOBULIN A IN TYPE 1 DIABETES
• Author: Matej Nemčić
• Mentor: Olga Gornik Kljaić (mentor)
• Committee member: Biljana Balen (predsjednik povjerenstva)
• Committee member: Toma Keser (član povjerenstva)
• Committee member: Dario Rahelić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2024-02-20, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 577 - Biochemistry. Molecular biology. Biophysics

Abstract

N glikozilacij a u šećernoj bolesti tipa 1 (ŠBT1) povezan a je s početkom bolesti , dijabetičkim
komplikacijama i lošom glikemijskom kontrolom , a osim navedenog, novija istraživanja
naglašavaju ulogu i munoglobulin a A (IgA) i njegove glikozilacije u autoimunim bolestima. U
ovom istraživanju je tekućinsk om kromatografij om ultra visoke djelotv ornosti analizirana
povezanost N glikoma ukupnih serumskih proteina te antitijela IgA s a ŠBT1 kako bi se pružio
uvid u obrasce N glikozilacije odraslih, identificirale promjene jedinstvene za početak bolesti
u djece , te ispitala povezanost N glikoma s komplikacijama i rizičnim faktorima N glikom
ukupnih serumskih proteina značajno se mijenja u ŠBT1 u obrazac nalik upalnim procesima te
pokazuje asocijacije s glikemijskom kontrolom i navikama pušenja, ali ne sa statusom
komplikacija Početak ŠBT1 u djece slabo se odražava na N glikom antitijela IgA, dok je ŠBT1
u odraslih povezan a s brojnim promjenama i povećanim grananjem N glika n a . Navedeno
upućuj e na uključenost N glikoma u različitim fazama ŠBT1 te na potencijalnu ulogu u
imunosnim odgovorima i upalnim procesima u ŠBT1

Abstract (english)

N-glycosylation in type 1 diabetes (T1D) is linked to disease onset, complications, and poor glycaemic control. In addition, recent findings emphasize the role of immunoglobulin A (IgA) and its glycosylation in autoimmune diseases. In this study, the association of N-glycome of total serum proteins and IgA with T1D was analysed by ultra-high performance liquid chromatography to elucidate the N-glycosylation patterns of adults, identify changes unique to the onset in children, and examine the association of N-glycome with complications and risk factors. N-glycome of total serum proteins significantly changes in T1D in an inflammatory-like pattern, and associates with glycaemic control and smoking, but not complications. The onset of T1D barely reflects in the N-glycome of IgA, while T1D in adults associates with substantial changes and increased branching. These findings indicate the involvement of N-glycome in different stages of T1D and a potential role in immune responses and inflammatory processes in T1D.

• Keywords: N glikozilacija; šećerna bolest tipa 1; serumski protein i; imunoglobulin A; komplikacije šećerne bolesti; HbA1c; pušenje
• Keywords (english): N-glycosylation; type 1 diabetes; total serum proteins; immunoglobulin A; diabetic complications; HbA1c; smoking
• Language: croatian
• URN:NBN: urn:nbn:hr:217:329296
• Promotion: 2024
• Project: Number: KK.01.2.2.03.0006; Title: Centar kompetencija u molekularnoj dijagnostici; Jurisdiction: eu; Funder: Europska komisija; Funding stream: Europski fond za regionalni razvoj
• Study programme: Title: Doctoral study; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti u području prirodnih znanosti (doktor/doktorica znanosti u području prirodnih znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-02-27 11:03:29