Molekularne značajke marokanskog virusa mozaika lubenice prvi put zabilježenog u Hrvatskoj

master's thesis

• Title (english): Molecular characteristics of Moroccan watermelon mosaic virus detected in Croatia for the first time
• Author: Marko Marohnić
• Mentor: Dijana Škorić (mentor)
• Committee member: Dijana Škorić (predsjednik povjerenstva)
• Committee member: Renata Šoštarić (član povjerenstva)
• Committee member: Mirta Tkalec (član povjerenstva)
• Committee member: Maja Matulić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2024-02-05, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Marokanski virus mozaika lubenice (eng. Morrocan watermelon mosaic virus, MWMV) pripadnik je roda Potyvirus unutar porodice Potyviridae. Ekonomski je značajan virus koji na zaraženim biljkama uzrokuje teške simptome i time dovodi do velikih gubitaka pri uzgoju tikvenjača (biljke roda Cucurbita). Virus je prvi put identificiran 1972. godine u Maroku, a do sada njegova prisutnost nije bila zabilježena u Hrvatskoj. Cilj ovog diplomskog rada bio je potvrditi infekciju MWMV-om na uzorcima simptomatičnih tikvenjača prikupljenih na području Pitomače 2021. i 2022. i time potvrditi prisutnost MWMV-a na teritoriju Republike Hrvatske. Također, cilj je bio i istražiti molekularne značajke izolata novozabilježenog virusa i utvrditi kojoj filogeografskoj skupini bi hrvatski izolati mogli pripadati. Metodom reverzne transkripcije i lančane reakcije polimerazom (RT-PCR) potvrđena je infekcija MWMV-om kod šest uzoraka tikvica, a filogenetskim analizama amplikona utvrđena je pripadnost hrvatskih izolata istoj filogeografskoj skupini u kojoj se nalaze izvorni izolati iz Maroka.

Abstract (english)

The Moroccan watermelon mosaic virus (MWMV), a member of the
Potyvirus genus within the Potyviridae family, is an economically significant virus that causes severe symptoms on infected
plants leading to substantial losses in the cultivation of cucurbits (plants of the Cucurbita genus).
The virus was first identified in Morocco in 1972 and has not been previously reported in Croatia.
The aim of this thesis was to confirm MWMV infection in symptomatic cucurbit samples collected
in the Pitoma č a region in 2021 and 2022, thereby confirming the presence of MWMV in the
territory of the Republic of Croatia. Additionally, the goal was to molecularly characterize the
newly detected virus isolates and determine its phylogeographic group ing . MWMV infection was
confirmed in six zucchini samples using the reverse transcription polymerase chain reaction (RT
PCR) method . P hylogenetic analysis of the amplicons revealed that the Croatian isolates belong
to the same phylogeographic group as the original isolates from Morocco.

• Keywords: MWMV; tikvica; RT-PCR; filogenija
• Keywords (english): MWMV; zucchini; RT PCR; phylogeny
• Language: croatian
• URN:NBN: urn:nbn:hr:217:805264
• Project: Number: IPS-2020-01-2960; Title: Visokoprotočno Nanopore-sekvenciranje mikrobnih genoma u rješavanju problema dijagnostike i epidemiologije biljnih patogena; Title: High-throughput Nanopore-sequencing of microbial genomes in solving problems of diagnosis and epidemiology of plant pathogens; Leader: Dijana Škorić; Jurisdiction: Croatia; Funder: HRZZ; Funding stream: IPS
• Study programme: Title: Molecular Biology-University graduate programme; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra molekularne biologije (sveučilišni/a magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2024-12-05
• Created on: 2024-02-27 11:31:53