Ekspresija proteina GLI1 i S100A7 u melanomu čovjeka

master's thesis

• Title (english): GLI1 and S100A7 protein expression in human melanoma
• Author: Helena Hajpek
• Mentor: Maja Sabol (mentor)
• Mentor: Inga Urlić (komentor)
• Committee member: Inga Urlić (predsjednik povjerenstva)
• Committee member: Petra Peharec Štefanić (član povjerenstva)
• Committee member: Sofia Ana Blažević (član povjerenstva)
• Committee member: Maja Matulić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2024-02-27, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Signalni put Hedgehog važan je tijekom embrionalnog razvoja, te u odraslom organizmu u održavanju homeostaze tkiva. Otkriće aberantne aktivacije puta Hedgehog kod melanoma, jednog od najagresivnijih tumora, značilo je i potencijalno usmjeravanja novih terapija i dijagnostike melanoma na komponente ovog puta i na gene i proteine koje ovaj signalni put regulira. U ovom diplomskom radu analizirana je ekspresija proteina GLI1 i S100A7 na uzorcima melanoma iz pacijenata te na staničnim linijama melanoma i keratinocita kože. Glavni cilj bio je ispitati postoji li povezanost u ekspresiji ova dva proteina u tkivima melanoma, kako bi se utvrdilo regulira li GLI1 ekspresiju S100A7. Ekspresija transkripcijskog faktora GLI1, komponente puta Hedgehog, i njegove mete, proteina S100A7, detektirani su uglavnom u epitelu koji okružuje tumor, dok je njihova ekspresija u samim tumorima slaba. Intenzitet bojanja oba proteina raste sa stadijem bolesti. Imunofluorescencija na staničnim linijama dodatno je potvrdila porast ekspresije proteina S100A7 u ovisnosti o ekspresiji proteina GLI1 staničnom modelu melanoma i keratinocita kože. Proteini GLI1 i S100A7 važna su komponenta razvoja melanoma pa bi nova istraživanja usmjerena na ove proteine mogla dovesti do njihovog korištenja kao potencijalnih prognostičkih markera, kao i do razvoja novih lijekova.

Abstract (english)

The Hedgehog signaling pathway is important during embryonic development, and in the adult organism in maintaining tissue homeostasis. The discovery of aberrant activation of the Hedgehog pathway in melanoma, one of the most aggressive tumors, meant potentially directing new therapies and melanoma diagnostics to the components of this pathway and to the genes and proteins that this signaling pathway regulates. In this graduate thesis, the expression of GLI1 and S100A7 proteins was analyzed on melanoma samples from patients and on melanoma and skin keratinocytes cell lines. The main objective was to examine whether there is a connection in the expression of these two proteins in melanoma tissues, in order to determine whether GLI1 regulates the expression of S100A7. The expression of the transcription factor GLI1, a component of the Hedgehog pathway, and its target, the protein S100A7, were detected mainly in the epithelium surrounding the tumor, while their expression in the tumors themselves was weak. The staining intensity of both proteins increases with the stage of the disease. Immunofluorescence on cell lines further confirmed the increase in S100A7 protein expression in dependence on GLI1 protein expression in melanoma and skin keratinocyte cell models. GLI1 and S100A7 proteins are an important component of melanoma development, so new research focused on these proteins could lead to their use as potential prognostic markers, as well as to the development of new drugs.

• Keywords: signalni put Hedgehog; epitel; imunohistokemija; imunofluorescencija
• Keywords (english): Hedgehog signaling pathway; epithelium; immunohistochemistry; immunofluorescency
• Language: croatian
• URN:NBN: urn:nbn:hr:217:768517
• Study programme: Title: Molecular Biology-University graduate programme; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra molekularne biologije (sveučilišni/a magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2024-08-27
• Created on: 2024-03-13 11:30:59