Povezanost polimorfizma gena FTO s tjelesnom masom i mikrobiomom usne šupljine u žena Zagrebačke županije

master's thesis

• Title (english): Association study of FTO gene single nucleotide polymorphism rs9939609 with body mass and oral microbiome in women from the Zagreb Region
• Author: Lucija Škara
• Mentor: Višnja Bačun-Družina (mentor)
• Mentor: Mirna Ćurković Perica (mentor)
• Committee member: Mirna Ćurković Perica (predsjednik povjerenstva)
• Committee member: Vesna Benković (član povjerenstva)
• Committee member: Željka Vidaković-Cifrek (član povjerenstva)
• Committee member: Inga Marijanović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2016-05-12, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Pretilost je rastući problem diljem svijeta. Nastaje kao posljedica kronične neravnoteže unosa i potrošnje energije. Etiologija pretilosti je vrlo složena, a procjenjuje se da su genetičke razlike među pojedincima uzrok 40 – 70% razlike u sklonosti razvoju pretilosti. Gen FTO (eng. Fat mass and obesity-associated) gen je s najvećim efektom na indeks tjelesne mase i rizik razvoja pretilosti. Cilj ovog rada je bio utvrditi razlike u distribuciji polimorfizma rs9939609 (AA, AT ili TT) gena FTO i razlike u sastavu mikroorganizama usne šupljine u pretilih osoba i osoba normalne tjelesne mase. Brojnim studijama na različitim populacijama potvrđeno je da je polimorfizam rs9939609 u prvom intronu gena FTO povezan s povećanom sklonošću razvoja pretilosti i dijabetesa tipa 2, a ovim istraživanjem i metodom lančane reakcije polimeraze u stvarnom vremenu ustanovljena je učestalost tog polimorfizma u žena Zagrebačke županije. Identifikacijom mikroorganizama iz sline ispitana je razlika mikrobioma dvije ispitivane skupine. Ispitanice su ispunile i upitnik koji je dao uvid u njihov način života, prehrane i bolesti te su ti podaci upotpunili rezultate genotipizacije i identifikacije bakterija usne šupljine. Dobiveni rezultati trebali bi pridonijeti boljem razumijevanju uloge polimorfizma rs9939609 gena FTO, mikrobioma usne šupljine i načina života u razvoju pretilosti na ovom području.

Abstract (english)

Obesity is a growing problem all over the world and results from chronic imbalance of energy intake and energy expenditure. The etiology of obesity is very complex. It is estimated that the genetic differences among individuals cause 40 - 70% of the difference in susceptibility to develop obesity. The gene FTO (eng. Fat mass and obesity-associated) is a gene with the largest effect on body mass index and the risk of developing obesity. The aim of this study is to determine the differences in the distribution of each genotype polymorphism rs9939609 (AA, AT or TT) and differences in oral microbiome between obese and normal weight individuals. Many studies on various populations confirmed association of the polymorphism rs9939609 in the first intron of the FTO gene with an increased susceptibility of obesity development and type 2 diabetes, and with this study by real-time PCR frequency of this polymorphism in women Zagreb County was determined. The difference in the microbiome was revealed by identification of the microorganisms from saliva of the two tested groups. Participants completed a questionnaire that gave insight into their lifestyle, diet and diseases and these data supplemented the results of genotyping and identification of bacteria of the oral cavity. The results of this study should contribute to a better understanding of the role of FTO gene polymorphism rs9939609, oral microbiome and lifestyle in the development of obesity in this area.

• Keywords: pretilost; gen FTO; rs9939609; mikroorganizmi usne šupljine
• Keywords (english): obesity; FTO gene; rs9939609; oral microbiome
• Language: croatian
• URN:NBN: urn:nbn:hr:217:275957
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-05-12 11:18:31