Utjecaj neravnoteže čimbenika oksidativnoga sustava na vaskularni endotelni faktor rasta

doctoral thesis

• Title (english): Oxidathive system factor imbalance impact on vascular endothelial growth factor
• Author: Vlatka Brzović Šarić
• Mentor: Domagoj Đikić (mentor)
• Mentor: Branimir Cerovski (sumentor); MBZ: 144926
• Committee member: Asija Stipić-Marković (član povjerenstva); MBZ: 70156
• Committee member: Vesna Benković (član povjerenstva)
• Committee member: Mirko Hadžija (član povjerenstva); MBZ: 15065
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2014, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 577 - Biochemistry. Molecular biology. Biophysics

Abstract

Povezanost između unutarstanične hiperglikemije, promjene u oksidacijskoredukcijskoj homeostazi, pojavnost oksidativnog stresa, smanjenje antioksidativne obrane i povećano stvaranje vaskularnoga endotelnog faktora rasta (VEGF), ključni su događaji u patogenezi proliferativne dijabetičke retinopatije (PDR). Ciljevi istraživanja: 1. Utvrditi koncentracije: VEGF-a, markera oksidativnog stresa i antioksidativnog sustava u staklastom tijelu i serumu. 2. Utvrditi korelaciju tih parametara u staklastom tijelu i serumu. 3. Temeljem rezultata korelativnog odnosa mjerenih pokusnih parametara, predvidjeti mogućnost dijagnostike retinopatičnih promjena mjerenjima serumskih vrijednosti. Metode istraživanja: ispitivane varijable mjerene su u uzorcima staklastog tijela i seruma bolesnika i eksperimentalnih životinja s izraženom proliferativnom dijabetičkom retinopatijom te u istim uzorcima bolesnika i eksperimentalnih životinja bez pojavnosti oksidativnog stresa, kao kontrola. Svi parametri analizirani su standardiziranim laboratorijskim metodama, a dobivene vrijednosti statistički obrađene. Korelacije podataka između promatranih skupina prikazane su Spearmanovim koeficijentima korelacije. Rezultati su pokazali povišene markere oksidativnog stresa u oku i u sistemskoj cirkulaciji, smanjen antioksidativni kapacitet u staklastom tijelu, te povišene vrijednosti u serumu kod dijabetičara s PDR-om. Zaključak: U dijabetičara, praćenjem serumskih parametara (lipidne peroksidacije, superoksid dismutaze i VEGF-a), moguće je indirektno procjenjivati oksidativni status unutar oka, a time i nastanak/napredovanje dijabetičke retinopatije i prije nego što promjene u oku postanu klinički očite.

Abstract (english)

The relationship between intracellular hyperglycemia, changes in oxidative - reductive homeostasis, the onset of oxidative stress, reduction of antioxidant defense and increased production of VEGF are considered to be key events in the pathogenesis of diabetic retinopathy. Research goals: 1. To determine concentrations of: vascular endothelial growth factor, markers of oxidative stress and antioxidant system in the vitreous humor and serum. 2. To determine the correlation between these parameters in the vitreous and serum. 3. Based on the results of the correlation of the measured experimental parameters, predict the ability to diagnose retinopathy changes based on serum values. Research Methods: The examined variables were measured in samples of vitreous and serum of patients and experimental animals with pronounced proliferative diabetic retinopathy and of control group of patients and experimental animals without incidence of oxidative stress. All parameters were analyzed by standardized laboratory methods and the obtained values were statistically evaluated. Data correlations between observed groups were presented by the Spearman correlation coefficient. Results: The results showed elevated markers of oxidative stress in the eye and systemic circulation, reduced antioxidant capacity in the vitreous humor and increased antioxidant capacity in diabetic patients with PDR. Conclusion: In diabetic patients, monitoring of serum parameters (Lipid Peroxidation, Superoxide Dismutase and VEGF), can be used to indirectly evaluate the oxidative status inside the eye, and thus the formation/progression of diabetic retinopathy before clinical manifestation of changes in the eye.

• Keywords: oksidativni stres; vaskularni endotelni faktor rasta; proliferativna dijabetička retinopatija
• Keywords (english): oxidative stress; vascular endothelial growth factor; proliferative diabetic retinopathy
• Language: croatian
• URN:NBN: urn:nbn:hr:217:359190
• Study programme: Title: Biology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-17 12:16:13