Genotipizacija izolata parvovirusa B19 iz epidemije u Hrvatskoj 2023. godine

master's thesis

• Title (english): Molecular typing of parvovirus B19 isolates from 2023. epidemic in Croatia
• Author: Laura Peček
• Mentor: Ivana Grgić (mentor)
• Mentor: Silvija Černi (komentor)
• Committee member: Silvija Černi (predsjednik povjerenstva)
• Committee member: Tomislav Ivanković (član povjerenstva)
• Committee member: Martina Šeruga Musić (član povjerenstva)
• Committee member: Zlatko Liber (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2024-09-18, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Parvovirus B19 jednolančani je DNA virus bez ovojnice iz porodice Parvoviridae, a jedini mu je domaćin čovjek. Primarno zaražava stanice prekursore eritrocita te se virusna replikacija odvija u jezgri zaražene stanice. Parvovirus B19 prenosi se kapljičnim putem, krvlju i krvnim derivatima te s majke na dijete, a uzrokuje bolest erythema infectiosum, koja se naziva još i „peta bolest“. Obolijevaju najčešće osobe pedijatrijske dobi koje razvijaju blage simptome, dok u trudnica, imunokompromitiranih pojedinaca i osoba s hematološkim poremećajima može izazvati težu kliničku sliku. Na temelju filogenetske analize regije NS1 virusnog genoma razlikujemo genotipove 1, 2 i 3. Genotipovi 1 i 3 dodatno su podijeljeni u podtipove A i B. Cilj ovog istraživanja bio je odrediti raspodjelu genotipova i podtipova parvovirusa B19 u Hrvatskoj. U istraživanje je bilo uključeno 107 ispitanika zaraženih parvovirusom B19 čiji su uzorci krvi u periodu od ožujka do rujna 2023. godine zaprimljeni u Kliniku za infektivne bolesti „Dr. Fran Mihaljević“. Sekvencije dobivene Sangerovom metodom sekvenciranja filogenetski su vrlo slične te pripadaju genotipu 1A. Nukleotidne supstitucije detektirane su u šest uzoraka, od čega pet istoznačnih i jedna zamjenska mutacija. Izrazita sličnost dobivenih sekvencija potvrđuje da je riječ o epidemiji, a ne o sporadičnim slučajevima zaraze.

Abstract (english)

Parvovirus B19 is an uncoated single-stranded DNA virus from the Parvoviridae family. This human pathogen primarily infects erythroid progenitors while viral replication occurs in the nucleus of the infected cell. Parvovirus B19 is transmitted through respiratory secretions, blood and blood products as well as vertically, causing fifth disease or erythema infectiosum. Pediatric patients most commonly develop mild symptoms, while pregnant women, immunocompromised individuals and those with hematological disorders may experience more severe clinical manifestations. Based on phylogenetic analysis of the NS1 region of the viral genome, genotypes 1, 2, and 3 are distinguished. Genotypes 1 and 3 are further divided into subtypes A and B. The aim of this study was to determine the distribution of parvovirus B19 genotypes and subtypes in Croatia. The study included 107 participants infected with parvovirus B19, whose blood samples were analyzed at the University Hospital for Infectious Diseases "Dr. Fran Mihaljević" between March and September 2023. The sequences obtained by the Sanger sequencing method showed high phylogenetic similarity and all clustered to genotype 1A. Nucleotide substitutions were detected in six samples, five of which were samesense and one was a missense mutation. The results suggest a common source of infection during the epidemic.

• Keywords: zaraza parvovirusom B19; genotip; sekvenciranje; molekularna filogenija
• Keywords (english): parvovirus B19 infection; genotype; sequencing; molecular phylogeny
• Language: croatian
• URN:NBN: urn:nbn:hr:217:648131
• Study programme: Title: Molecular Biology-University graduate programme; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra molekularne biologije (sveučilišni/a magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-09-30 10:39:12