Karakterizacija kromatinskih nestabilnosti u limfocitima čovjeka uslijed izloženosti niskim koncentracijama pesticida

doctoral thesis

• Title (english): Characterization of chromatin instabilities in human lymphocytes due to low concentration of pesticide exposure
• Author: Marin Mladinić
• Mentor: Davor Želježić (mentor)
• Committee member: Mirjana Pavlica (predsjednik povjerenstva)
• Committee member: Davor Želježić (član povjerenstva)
• Committee member: Vesna Benković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2012-06-12, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 577 - Biochemistry. Molecular biology. Biophysics

Abstract

Dosadašnja istraživanja ukazala su da izloženost pesticidima može uzrokovati genetička oštećenja i povećati rizik od razvoja neoplazija. Istraživanje genotoksičnosti pesticida provedeno je u uvjetima ex vivo na primarnim i dugotrajnim kulturama limfocita čovjeka, te in vivo na limfocitima osoba profesionalno izloženih pesticidima glifosatu, terbutilazinu i karbofuranu. Terbutilazin i karbofuran s i bez metaboličke aktivacije pokazali su komet testom genotoksični učinak u 4-satnom izlaganju, dok... More to nije zapaženo za glifosat. Mikronukleus testom uz primjenu fluorescencijske hibridizacije in situ dokazano je da mikronukleusi sadrže i cijele kromosome čime ispoljavaju i aneugeni mehanizam djelovanja. Metabolički promijenjen terbutilazin pokazao je ograničeni specifičan aneugeni učinak na kromosome 1, 9, 15, 16 i Y. U 14-dnevnom izlaganju uočeni genotoksični potencijal pesticida bio je izraženiji u odnosu na kratkotrajno izlaganje. Primjenom komet-FISH-a, nakon dugotrajne izloženosti utvrđena je povećana strukturna nestabilnost gena c-Myc i TP 53. Formulacije pesticida pokazale su Less

Abstract (english)

Up to date research have shown an association between pesticide exposure and genome damage as well as elevated risk of cancer. Present genotoxicity research was performed on primary and long-term lymphocyte cultures ex vivo and will comprise occupationally exposed populations. Genotoxic effect of terbuthylazine and carbofuran exposure was observed with and without metabolic activation by comet assay, while this was not the case for glyphosate exposure. Fluorescent-in-situ-hybridization-coupled... More micronucleus test showed that micronuclei contain fragments and whole chromosomes indicating clastogenic but also aneugenic mechanism of action. Metabolically transformed terbuthylazine induced a limited specific aneugenic effect on chromosomes 1, 9, 15, 16 and Y. Increased structural instability of genes c-Myc and TP 53 was observed using comet-FISH after prolonged pesticide exposure. Pesticide formulations proved to increase genome instability compared to pure active ingredients. Less

• Keywords: pesticidi; komet test; mikronukleus test; FISH; komet-FISH; dugotrajna kultura limfocita; profesionalna izloženost
• Keywords (english): pesticides; comet assay; micronucleus assay; FISH; comet-FISH; extended-term lymphocyte cultures; professional exposure
• Language: croatian
• URN:NBN: urn:nbn:hr:217:009635
• Project: Number: 022-0222148-2137; Title: Genotoksičnost kemijskih i fizikalnih agensa prirodnog i antropogenog podrijetla; Title: Genotoxicity of chemical and physical agents of natural and anthropogenic origin; Leader: Vilena Kašuba; Jurisdiction: Croatia; Funder: Ministarstvo znanosti, obrazovanja i mladih Republike Hrvatske; Funding stream: zProjects
• Study programme: Title: Doctoral study; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti u području prirodnih znanosti (doktor/doktorica znanosti u području prirodnih znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2025-01-27 09:34:18