Promjena ekspresije gena REL, PDL1 i CXCL10 faktorom nekroze tumora alfa i interferonom gama u stanicama karcinoma vrata maternice i primarnog medijastinalnog B-staničnog limfoma čovjeka

master's thesis

• Title (english): Tumor Necrosis Factor-alpha and Interferon-gama induced changes of REL, PDL1, and CXCL10 expression in human cervical carcinoma and primary mediastinal B-cell lymphoma cell lines
• Author: Valentino Mihalić
• Mentor: Petra Korać (mentor)
• Mentor: Paula Gršković (komentor)
• Committee member: Petra Korać (predsjednik povjerenstva)
• Committee member: Inga Urlić (član povjerenstva)
• Committee member: Tomislav Ivanković (član povjerenstva)
• Committee member: Željka Vidaković-Cifrek (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2024-09-19, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Primarni medijastinalni B-stanični limfom (PMBCL, od eng. primary mediastinal B-cell
lymphoma), limfom koji dijeli neke značajke s difuznim velikim B-staničnim limfomom
(DLBCL, od eng. diffuse large B-cell lymphoma) i klasičnim Hodgkinovim limfomom (cHL,
od eng. classical Hodgkin lymphoma), obilježen je konstitutivnom aktivnošću signalnih putova
NF-κB i JAK-STAT, koji dovode do transkripcije gena uključenih u imunosnu modulaciju i
preživljavanje stanica. Cilj ovog istraživanja bio je analizirati obrasce ekspresije specifičnih
gena u staničnim linijama PMBCL-a MedB-1 i Karpas 1106p te u staničnoj liniji HeLa, koja
potječe iz ljudskog karcinoma vrata maternice, nakon tretmana različitim dozama proupalnih
citokina TNF-α i IFN-γ. Osnovne razine ekspresije gena REL, CXCL10 i PDL1 bile su više u
staničnim linijama modelima PMBCL-a nego u stanicama HeLa. 48 sati nakon tretmana
citokinom TNF-α, stanice MedB-1 pokazale su značajno povećanje ekspresije gena REL i PDL1
pri dozama od 10, 20 i 50 ng/ml. Stanice HeLa pokazale su značajno povećanje ekspresije gena
CXCL10 24 sata nakon tretmana s 5, 10 i 20 ng/ml TNF-α, za razliku od stanica MedB-1 i
Karpas 1106p, koje nisu pokazale promjene u ekspresiji gena CXCL10. Nakon tretmana IFN-
γ, sve stanične linije pokazale su značajno povećanje ekspresije gena CXCL10 i PDL1, pri čemu
su stanice HeLa pokazale najveći porast ekspresije gena PDL1.

Abstract (english)

Primary mediastinal B-cell lymphoma (PMBCL), a type of lymphoma that shares some features
with diffuse large B-cell lymphoma (DLBCL) and classical Hodgkin lymphoma (cHL), is
marked by constitutive activity of the NF-κB and JAK-STAT signaling pathways, which lead
to the transcription of genes involved in immune modulation and cell survival. The aim of this
research was to analyze the expression patterns of specific genes in the PMBCL cell lines
MedB-1 and Karpas 1106p and in the HeLa cell line, which originates from human cervical
cancer, after treatment with different doses of the pro-inflammatory cytokines TNF-α and IFN-
γ. Baseline gene expression levels of REL, CXCL10, and PDL1 were higher in PMBCL-derived
cell lines than in HeLa cells. 48 hours after TNF-α treatment, MedB-1 cells showed a significant
increase in REL and PDL1 gene expression at doses of 10, 20 and 50 ng/ml. HeLa cells showed
a significant increase in CXCL10 gene expression 24 hours after treatment with 5, 10 and 20
ng/ml TNF-α, in contrast to MedB-1 and Karpas 1106p cells, which did not show changes in
CXCL10 gene expression. After IFN-γ treatment, all cell lines showed a significant increase in
CXCL10 and PDL1 gene expression, with HeLa cells showing the highest increase in PDL1
gene expression.

• Keywords: MedB-1; Karpas 1106p; HeLa; NF-κB; JAK-STAT
• Keywords (english): MedB-1; Karpas 1106p; HeLa; NF-κB; JAK-STAT
• Language: croatian
• URN:NBN: urn:nbn:hr:217:344224
• Study programme: Title: Molecular Biology-University graduate programme; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra molekularne biologije (sveučilišni/a magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2025-01-29 14:41:41