Terapijski učinak probiotičkog soja Limosilactobacillus fermentum (MC1) i njegovih egzopolisaharida na kolitis izazvan natrij dekstran sulfatom u ženki miša

master's thesis

• Title (english): Therapeutic effect of the probiotic strain Limosilactobacillus fermentum (MC1) and its exopolysaccharides on colitis induced by sodium dextran sulfate in female mice
• Author: Sara Bilić
• Mentor: Nada Oršolić (mentor)
• Mentor: Dyana Odeh (komentor)
• Committee member: Nada Oršolić (predsjednik povjerenstva)
• Committee member: Vesna Benković (član povjerenstva)
• Committee member: Tomislav Ivanković (član povjerenstva)
• Committee member: Domagoj Đikić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2024-12-17, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Ulcerozni kolitis je kronična, multifaktorna upalna bolest crijeva za koju je karakteristična narušenost crijevne mikrobiote, što pridonosi progresiji upale i razvoju oksidacijskog stresa. Crijevna mikrobiota utječe na lokalne i sustavne imunosne odgovore, zbog čega se istražuje kao moguća terapija u liječenju ulceroznog kolitisa. Cilj rada bio je istražiti terapijski učinak probiotičkog soja bakterije Limosilactobacillus fermentum MC1 i njegovih egzopolisaharida (EPS) na kolitis izazvan natrij dekstran sulfatom (DSS) u miševa kroz praćenje polarizacije makrofaga, patohistoloških, biokemijskih i antioksidacijskih promjena tkiva. Sažimajući podatke možemo zaključiti da probiotički soj bakterije L. fermentum MC1 i njegovi EPS posjeduju protuupalna i antioksidacijska svojstva koja doprinose smanjenju upalnih procesa kod kolitisa u ženki miševa soja C57BL/6, što potvrđuju slijedeći pokazatelji: smanjeni indeks bolesti, smanjeno krvarenje, povećanje hematopoetskih pokazatelja i nedostatak biokemijskih promjena te polarizacija makrofaga prema M2 fenotipu povezana sa smanjenjem upale i displazije uz povećanje popravka i obnove crijevne sluznice te održavanje homeostaze tkiva debelog crijeva. Dobiveni podaci kao i poboljšanje histopatologije tkiva debelog crijeva daju osnovu za daljnja istraživanja moguće primjene probiotičkog soja bakterije L. fermentum MC1 i njegovih EPS u terapiji kolitisa.

Abstract (english)

Ulcerative colitis is a chronic, multifactorial inflammatory bowel disease characterised by a disrupted gut microbiota, contributing to inflammation progression and the development of oxidative stress. The gut microbiota influences both local and systemic immune response, making it a focus of investigation as a potential therapy for ulcerative colitis. The aim of this thesis was to investigate the therapeutic effect of the probiotic strain Limosilactobacillus fermentum MC1 and its exopolysaccharides (EPS) on sodium dextran sulfate (DSS)-induced colitis in mice by monitoring macrophage polarization, pathohistological, biochemical and antioxidative tissue changes. The findings indicate that the probiotic strain L. fermentum MC1 and its EPS exhibit anti-inflammatory and antioxidative properties, which contribute to alleviate inflammatory activity and reduce damage in DSS-induced colitis in female C57BL/6 mice. This is supported by the following indicators: reduced disease activity index, decreased bleeding, increased hematopoietic parameters, absence of significant biochemical changes, and macrophage polarization toward the M2 phenotype associated with reduced inflammation and dysplasia, enhanced repair and regeneration of the intestinal mucosa, and maintenance of colon tissue homeostasis. The obtained data, along with improved histopathological findings in colon tissue, provide the basis for further research into the possible application of L. fermentum MC1 and its EPS in colitis therapy.

• Keywords: riječi: probiotici; egzopolisaharidi; ulcerozni kolitis; natrij dekstran sulfat; oksidacijski stres
• Keywords (english): probiotics; exopolysaccharides; ulcerative colitis; sodium dextran sulfate; oxidative stress
• Language: croatian
• URN:NBN: urn:nbn:hr:217:136792
• Project: Number: IP-2019-04-2237; Title: Potencijalne terapijske biomolekule druge generacije probiotika; Title: Potential therapeutic biomolecules of next-generation probiotics; Acronym: PRO-BIO 2.0; Leader: Blaženka Kos; Jurisdiction: Croatia; Funder: Hrvatska zaklada za znanost; Funding stream: Research Projects
• Study programme: Title: Experimental Biology-University graduate programme; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra eksperimentalne biologije (sveučilišni/a magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2025-02-13 08:28:49