Histomorfometrija i histopatologija kamenog koralja Cladocora caespitosa u Jadranskom moru

master's thesis

• Title (english): Histomorphometry and histopathology of the stone coral Cladocora caespitosa in the Adriatic sea
• Author: Sanja Krcivoj
• Mentor: Romana Gračan (mentor)
• Mentor: Pavel Ankon (komentor)
• Committee member: Romana Gračan (predsjednik povjerenstva)
• Committee member: Damir Sirovina (član povjerenstva)
• Committee member: Nenad Judaš (član povjerenstva)
• Committee member: Mirta Rubčić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2024-11-19, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Povišena temperatura mora uzrokovana klimatskim promjenama dovodi do oštećenja, nekroze, izbjeljivanja i sve češćeg odumiranja organizama kao što su koralji. Cilj ovog istraživanja bio je opisati histološko stanje zdravih, nekrotičnih i izbijeljenih polipa busenastog kamenog koralja, Cladocora caespitosa, te utvrditi postoje li razlike u građi tkiva i histopatološke promjene s obzirom na dvije istraživane lokacije (NP Mljet i Piranski zaljev) u Jadranskom moru. Uzorci tkiva su uklopljeni u... More paraplastne blokove, rezani na mikrotomu te obojeni hematoksilin-eozin metodom i metodom bojenja po Gramu. Uzorci bazalne i površinske stijenke tijela su pregledani pod svjetlosnim mikroskopom, te morfometrijski analizirani.Opisana je uredna/zdrava struktura tkiva kod zdravih polipa, te histopatološka oštećenja utvrđena kod nekrotičnih i izbijeljenih polipa. Mikroskopskom analizom histoloških preparata između dvije istraživane lokacije nisu utvrđene razlike u strukturi tkiva zdravih jedinki, niti u tipu utvrđenih lezija u nekrotičnim i izbijeljenim polipima, dok su histomorfometrijske usporedbe pokazale da postoje razlike u debljini epiderme i mezogleje unutar istih zdravstvenih stanja između dvije lokacije. S obzirom da ova vrsta izgrađuje koraljne grebene o kojem ovisi veliki broj vrsta, a podložna je utjecaju klimatskih promjena, ovakva istraživanja pridonose planiranju njene zaštite, ali i očuvanju morskih sustava u Sredozemnom moru. Less

Abstract (english)

Increased sea temperature caused by climate change leads to damage, necrosis, bleaching and increased mortality of organisms such as corals. The aim of research was to describe histological condition of healthy, necrotic and bleached polyps of stone coral Cladocora caespitosa and to explore possible differences in histological structures and histopathology between the two investigated locations in the Adriatic Sea (NP Mljet and Piran Bay). Tissues were embedded in paraplast blocks, cut on... More a microtome and stained with the hematoxylin-eosin and the Gram staining method. Slides from the basal and surface body wall were examined under a light microscope and morphometrically analysed. Histological architecture of healthy, necrotic and bleached polyps was described. There was no difference in histological structures of healthy individuals, nor in the type of lesions recorded in necrotic and bleached polyps between two locations. However, histomorphometric comparison showed that there are differences in the thickness of the epidermis and mesoglea within the same health condition between the two locations. Since the species is a reef building coral who shapes the habitat for numerous reef-associated organisms, and susceptible to climate change, this research contributes to its protection, but also to the conservation of marine ecosystems in the Mediterranean Sea. Less

• Keywords: izbjeljivanje; nekroza; simbiotski dinoflagelati; koraljni grebeni
• Keywords (english): bleach; necrosis; symbiotic dinoflagellates; coral reefs
• Language: croatian
• URN:NBN: urn:nbn:hr:217:017156
• Project: Number: IP-2019-04-3389; Title: Utjecaj klimatskih promjena na bioraznolikost koralja - istraživanje slučaja masovnih ugibanja u Jadranskom moru; Title: Impact of climate change on coral biodiversity - research of mass mortality events in the Adriatic Sea; Acronym: ADRICOR; Leader: Petar Kružić; Jurisdiction: Croatia; Funder: Hrvatska zaklada za znanost; Funding stream: Research Projects
• Study programme: Title: Biology and Chemistry Education-University integrated undergraduate and graduate programme; specializations in: Biology; Course: Biology; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: sveučilišni/a magistar/magistra edukacije biologije i kemije (sveučilišni/a magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2025-02-25 14:20:28