Uloga jasmonata u regulaciji antioksidacijskog odgovora tijekom infekcije krumpira (Solanum tuberosum) viroidom vretenastog gomolja krumpira

master's thesis

• Title (english): The role of jasmonates in regulation of antioxidative response during potato spindle tuber viroid infection of potato (Solanum tuberosum)
• Author: Nina Lulić
• Mentor: Snježana Mihaljević (mentor)
• Mentor: Željka Vidaković-Cifrek (komentor)
• Committee member: Željka Vidaković-Cifrek (predsjednik povjerenstva)
• Committee member: Martina Šeruga Musić (član povjerenstva)
• Committee member: Nenad Malenica (član povjerenstva)
• Committee member: Mirta Tkalec (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2024-11-22, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Krumpir je jedna od najvažnijih usjevnih biljaka u svijetu. Biotički stres izazvan patogenima može
uzrokovati oksidacijski stres u napadnutim biljkama i velike gubitke u prinosu. Istražen je učinak
infekcije viroidom PSTVd na nakupljanje H2O2 i ekspresiju gena za antioksidacijske enzime,
peroksidaze klase III, i marker-gene za biosintezu i signalizaciju posredovanu jasmonskom
kiselinom (JA) u listovima biljaka divljeg tipa (WT), u JA-deficijentnim biljkama linije opr3 i JAneosjetljivim biljkama linije coi1. Metodom spektrofotometrije dokazano je pojačano nakupljanje
H2O2 u inficiranim biljkama WT, slabo nakupljanje H2O2 u inficiranim JA-neosjetljivim i
smanjeno nakupljanje H2O2 u inficiranim JA-deficijentnim biljkama, u 6. tjednu nakon inokulacije.
Metodom RTqPCR potvrđena je transkripcijska aktivacija gena za antioksidacijske enzime koja je
bila najjača u JA-deficijentnim biljkama, blago pojačana u WT, a smanjena u JA-neosjetljivim
biljkama. Tretman metil jasmonatom (MeJA) prije infekcije doveo je do smanjenog nakupljanja
H2O2 i ekspresije gena za antioksidacijske enzime u zaraženim biljkama WT, a pojačanog
nakupljanja H2O2 i ranije ekspresije gena u JA-deficijentnim biljkama, što ukazuje na ulogu JA u
regulaciji antioksidacijskih odgovora. Međutim, u sve tri ispitane linije krumpira, tretman s MeJA
nije značajnije utjecao na smanjenje osjetljivosti na PSTVd.

Abstract (english)

Potato is one of the most important crop plants worldwide. Biotic stress caused by pathogen attack
can cause oxidative stress in plants and result in great losses in yield. The effect of PSTVd infection
of potato plants on the accumulation of H2O2 as well as on the expression of genes encoding
antioxidant enzymes, class III peroxidases, and marker genes for jasmonic acid (JA) biosynthesis
and signaling was investigated in wild-type (WT) plants, JA-deficient opr3 plants and JAinsensitive coi1 plants. Spectrophotometric analysis indicated increased accumulation of H2O2 in
infected WT and coi1 plants, and decreased accumulation of H2O2 in infected opr3 plants, 6 weeks
after inoculation. RTqPCR analysis confirmed that transcriptional activation of genes encoding
antioxidant enzymes was strongest in JA-deficient plants, slightly enhanced in WT, and decreased
in JA-insensitive plants. Treatment with methyl jasmonate (MeJA) prior to infection led to reduced
H2O2 accumulation and transcriptional activation of genes for antioxidant enzymes in WT plants,
and increased accumulation of H2O2 and earlier gene expression in JA-deficient plants, which
indicates the role of JA in the regulation of antioxidant responses. However, MeJA did not reduce
the sensitivity of tested potato lines to PSTVd.

• Keywords: biotički stres; H2O2; metil jasmonat; peroksidaze klase III; PSTVd; viroid
• Keywords (english): biotic stress; class III peroxidases; H2O2; methyl jasmonate; PSTVd; viroid
• Language: croatian
• URN:NBN: urn:nbn:hr:217:073725
• Project: Number: IP-2019-04-9915; Title: Integrativna analiza signalnih puteva fitohormona uključenih u odgovor biljaka krumpira na infekciju viroidom vretenastoga gomolja krumpira; Title: Integrative analysis of phytohormone signaling pathways involved in potato response to infection with Potato spindle tuber viroid; Acronym: PotatoSignalHub; Leader: Snježana Mihaljević; Jurisdiction: Croatia; Funder: Hrvatska zaklada za znanost; Funding stream: Research Projects
• Study programme: Title: Molecular Biology-University graduate programme; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra molekularne biologije (sveučilišni/a magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2025-02-27 10:16:19