Kvantitativna ekspresija gena NAC041, NAC084, DREB2A i HSFA2 u klijanaca divljeg kupusa (Brassica incana) izloženih abiotičkom stresu

master's thesis

• Title (english): Quantification of NAC041, NAC084, DREB2A and HSFA2 gene expression in wild cabbage (Brassica incana) exposed to abiotic stress
• Author: Josipa Drmić
• Mentor: Nataša Bauer (mentor)
• Mentor: Sandra Vitko (komentor)
• Committee member: Nataša Bauer (predsjednik povjerenstva)
• Committee member: Dunja Leljak-Levanić (član povjerenstva)
• Committee member: Željka Vidaković-Cifrek (član povjerenstva)
• Committee member: Mirta Tkalec (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2025-02-07, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Kao posljedica klimatske krize, čimbenici abiotičkog stresa poput povišene temperature, suše i
povišenog saliniteta tla negativno utječu na biljni rast i razvoj, što ostavlja značajne posljedice na
prinos i kvalitetu poljoprivrednih kultura. Otkrivanje složenih mehanizama biljnog odgovora na
stres, razvoj strategija za povećanje otpornosti poljoprivrednih kultura te očuvanje genetičkih
resursa ključni su koraci za osiguranje globalne prehrambene sigurnosti. Mnoge divlje vrste iz roda
Brassica iznimno su otporne na stresne uvjete okoliša u kojima prirodno rastu, stoga predstavljaju
bitan izvor poželjnih svojstava za povećanje genetičke raznolikosti u poljoprivrednim vrstama
kupusnjača. U ovom radu predstavljena je analiza kvantitativne ekspresije četiriju gena uključenih
u biljni odgovor na abiotički stres NAC041, NAC084, DREB2A i HSFA2 u klijanaca divljeg kupusa
(Brassica incana) izloženih različitim čimbenicima abiotičkog stresa: povišenoj temperaturi,
osmotskom stresu, povišenom salinitetu te kombiniranom abiotičkom stresu. Značajno povišenje
ekspresije gena DREB2A i HSFA2 u dvije linije potvrdilo je bitnu ulogu ovih transkripcijskih
faktora u odgovoru divljeg kupusa na abiotički stres, dok su druge dvije linije pokazale značajno
manju promjenu ekspresije, što ukazuje na mogućnost postojanja više genskih varijanti. Utvrđeno
je smanjenje ekspresije gena NAC041 pod utjecajem osmotskog stresa, dok je značajno smanjenje
ekspresije gena NAC084 utvrđeno pod utjecajem visoke temperature i kombiniranog abiotičkog
stresa.

Abstract (english)

As a result of an ongoing climate crisis, abiotic stress factors such as elevated temperature, drought,
and increased soil salinity negatively affect plant growth and development, causing damage to the
yield and quality of agricultural crops. Unraveling the complex stress response mechanisms in
plants, developing crop improvement strategies, and undertaking germplasm conservation efforts
are key steps to ensure global food security. In an attempt to reintroduce genetic diversity into
Brassica crops, crop wild relative species are studied for their valuable stress resistance traits. This
study presents a quantitative gene expression analysis of the stress-responsive NAC041, NAC084,
DREB2A, and HSFA2 genes in wild cabbage (Brassica incana) exposed to different abiotic stress
treatments, including high-temperature stress, osmotic stress, salt stress, and combined abiotic
stress. A significant increase in DREB2A and HSFA2 expression in two accessions confirmed the
important role of these transcription factors in B. incana response to abiotic stress, while the other
two accessions showed more moderate expression changes, implying the possible presence of
multiple gene variants. A decrease in NAC041 expression was observed in one accession under
osmotic stress, while a substantial decrease in NAC084 expression was observed under heat stress
and combined stress in all four B. incana accessions.

• Keywords: Brassicaceae; divlje vrste srodne poljoprivrednim kulturama; biljni odgovor na stres; temperatura; suša; salinitet
• Keywords (english): Brassicaceae; crop wild relatives; plant stress response; temperature; drought; salinity
• Language: english
• URN:NBN: urn:nbn:hr:217:977540
• Project: Number: KK.05.1.1.02.0005; Title: Agrobioraznolikost - osnova za prilagodbu i ublažavanje posljedica klimatskih promjena u poljoprivredi; Title: Agrobiodiversity - the basis for adapting and mitigating the effects of climate change in agriculture; Leader: Smiljana Goreta Ban; Jurisdiction: eu; Funder: Europska unija; Funding stream: European Regional Development Fund
• Study programme: Title: Molecular Biology-University graduate programme; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra molekularne biologije (sveučilišni/a magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2025-02-27 13:12:51