Djelovanje postbiotika na modelu upalne bolesti crijeva in vitro

master's thesis

• Title (english): The effect of postbiotics on in vitro model of inflammatory bowel disease
• Author: Marta Protrka
• Mentor: Inga Urlić (mentor)
• Committee member: Inga Urlić (predsjednik povjerenstva)
• Committee member: Petra Korać (član povjerenstva)
• Committee member: Maja Matulić (član povjerenstva)
• Committee member: Tomislav Ivanković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2025-02-19, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Fermentirani čaj kombucha se konzumira tisućama godina i prepisuju mu se mnoga ljekovita
svojstva. Dosad je dokazano kako ima značajan antioksidacijski učinak i potencijalno pozitivno
djelovanje na mnoge bolesti od kojih su neke i upalne bolesti crijeva. S obzirom da za upalne
bolesti crijeva ne postoji terapija koja dovodi do izlječenja, istražuju se novi faktori uključeni u
patologiju bolesti kao što je oksidacijski stres i očuvanje epitelne barijere probavnog sustava.
Ovaj diplomski rad... More istražuje učinak postbiotika kombuche na ljudskom staničnom modelu kokulture staničnih linija Caco-2 i THP-1. Uspostavila sam model zdrave epitelne membrane i
model epitelne membrane u stanju upale te ih tretirala postbiotikom kombuche u različitim
testnim skupinama koje ispituju preventivni i terapijski učinak kombuche. Mjerenjem
transepitelnog otpora sam pokazala da kombucha jača integritet epitelne membrane crijeva.
Mjerenjem razine reaktivnih vrsta kisika nisam pokazala statistički značajan učinak na povećanje
niti smanjenje oksidacijskog stresa. Međutim, analizom ekspresije gena za enzime oksidacijskog
stresa pokazala sam da ih stanje upale blago pojačava i da tretman samo kombuchom izrazito
povećava ekspresiju katalaze (CAT) i superoksidne dismutaze 1 (SOD1). Rezultati istraživanja
upućuju na potencijalno bolji integritet crijevne barijere i veću spremnost obrane od
oksidacijskog stresa pri konzumaciji fermentiranog čaja kombuche. Less

Abstract (english)

Fermented kombucha tea has been consumed for thousands of years and is thought to have
healing properties. It has been confirmed to have a significant antioxidative effect and potentially
positive effect on many diseases, among which is Inflammatory bowel disease (IBD). Current
IBD therapies do not offer a complete cure for the disease, so new targets such as oxidative stress
and gut-barrier disruption are being investigated. This master thesis focuses on the effect of
kombucha... More postbiotics on a human cell co-culture model using Caco-2 and THP-1 cell lines. I
established a healthy co-culture and an inflamed one and I treated them both with the postbiotic
in different test groups which investigate kombucha’s preventative and therapeutic potential. By
measuring the transepithelial electrical resistance (TEER), I showed that the postbiotic has an
influence on improving barrier integrity. Measurements of reactive oxygen species did not show
statistically significant decrease nor increase in oxidative stress. But gene expression analysis of
antioxidant enzymes showed that inflammation causes a slight increase in their expression and
that treatment with kombucha alone, causes a drastic increase in expression of catalase (CAT)
and superoxide dismutase 1 (SOD1). Overall, the findings suggest that consuming fermented
kombucha tea may improve the gut barrier integrity and enhance the body's defense against
oxidative stress. Less

• Keywords: kombucha; IBD; oksidacijski stres; antioksidacijski enzimi; epitelna membrana
• Keywords (english): kombucha; IBD; oxidative stress; antioxidant enzymes; epithelial membrane
• Language: croatian
• URN:NBN: urn:nbn:hr:217:766968
• Study programme: Title: Molecular Biology-University graduate programme; Study programme type: university; Study level: graduate; Academic / professional title: sveučilišni/a magistar/magistra molekularne biologije (sveučilišni/a magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2025-02-28 13:07:34