Utjecaj imunosnoga sustava na prisutnost cirkulirajućih melanomskih stanica

doctoral thesis

• Title (english): Impact of immune system on presence of circulating melanoma cells
• Author: Jasmina Marić Brozić
• Mentor: Ivan Šamija (mentor); MBZ: 266224
• Committee member: Zvonko Kusić (član povjerenstva); MBZ: 119493
• Committee member: Nada Oršolić (član povjerenstva)
• Committee member: Ivan Šamija (član povjerenstva); MBZ: 266224
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2013, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 577 - Biochemistry. Molecular biology. Biophysics

Abstract

Metoda RT-PCR specifična za tirozinazu je visoko specifična i osjetljiva metoda otkrivanja cirkulirajućih melanomskih stanica (CMS). Iako CMS mogu biti cilj specifične imunološke reakcije protiv tumora, povezanost između imunokompetencije i prisutnosti CMS nije dosad istraživana. Cilj ovog rada je istražiti postoji li povezanost između prisutnosti CMS i različitih parametara imunološkog statusa. Analizirani su uzorci krvi 131 bolesnika s melanomom. Prisutnost melanomskih stanica u uzorcima krvi određena je metodom RT-PCR specifičnom za tirozinazu. Udio različitih skupina limfocita u uzorcima krvi kao parametar imunološkog statusa određen je protočnom citometrijom. Aktivnost stanica NK i fagocitne funkcije granulocita i monocita analizirani su kao funkcionalni parametri imunološkog statusa. Pokazana je visoka specifičnost i nizak prag osjetljivosti tirozinaze kao biljega za određivanje CMS. Bolesnici s pozitivnom vrijednošću tirozinaze imali su značajno kraće preživljenje čime sam potvrdila prognostičku vrijednost tirozinaze. Nije nađena povezanost između prisutnosti CMS i istraživanih parametara imunološkog statusa.

Abstract (english)

Tyrosinase-specific RT-PCR is highly sensitive and specific method for detection of circulating melanoma cells (CMC). Although CMC might be target of tumor-specific immune reaction, correlation between immunocompetence and presence of CMC hasn’t been studied so far. The aim of this study was to investigate if there is an association between the presence of CMC and different parameters of immune status. Blood samples from 131 patients with melanoma were analyzed. The presence of CMC was analyzed by tyrosinase-specific RT-PCR. Proportions of lymphocyte subsets in blood samples as a parameter of immune status were determined by flow-cytometry. Activity of NK cells and phagocytic functions of granulocytes and monocytes were analyzed as functional parameters of immune status. High specificity and low threshold for detection were shown for tyrosinase as a marker for CMC detection by RT-PCR. Patients positive for tyrosinase had significantly shorter survival, which confirms its prognostic value. No association between the presence of CMC and parameters of immune status was found.

• Keywords: melanom; imunološki status; tirozinaza; tumorski biljezi; cirkulirajuće tumorske stanice; tumorska imunost
• Keywords (english): melanoma; immune status; tyrosinase; tumor markers; circulating tumor cells; tumor immunity
• Language: croatian
• URN:NBN: urn:nbn:hr:217:306593
• Study programme: Title: Biology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-24 08:56:50