Morfološke, genetske i fitokemijske značajke populacije rogača (Ceratonia siliqua L.) u Hrvatskoj

doctoral thesis

• Title (english): Morphological, genetic and phytochemical traits of carob populations (Ceratonia siliqua L.) in Croatia
• Author: Dragojević Müller Ivna
• Mentor: Dario Kremer (mentor); MBZ: 240541
• Committee member: Gordana Rusak (predsjednik povjerenstva)
• Granter: University of Zagreb; Faculty of Science; Zagreb
• Defense date and country: 2017, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 57 - Biological sciences. Physical anthropology. Biodiversity

Abstract

Obzirom na oskudnost saznanja o hrvatskim populacijama rogača (Ceratonia siliqua L.), nutritivno i protektivno vrlo kvalitetne biljke, provedeno je istraživanje morfoloških, genetskih i fitokemijskih značajki populacija rogača u Hrvatskoj. Na sakupljenim uzorcima ploda i lista rogača napravljena su morfometrijska istraživanja, dok je genetska varijabilnost utvrđena primjenom AFLP analize genomske DNA izolirane iz lista. Udio ukupnih fenola, flavonoida i tanina te antioksidativni potencijal ploda i lista određen je spektofotometrijskim metodama. Udio makro i mikroelemenata određen je primjenom ICP-AES tehnike. Utvrđena je statistički značajna varijabilnost morfoloških i fitokemijskih značajka ploda i lista istraživanih populacija rogača. Molekularna AFLP analiza pokazuje značajnu varijabilnost istraživanih populacija, obzirom da je varijanca između individua unutar populacija znatno veća (75,51%) od varijance između populacija. Usprkos statistički značajnoj varijabilnosti UPGMA klaster analizom, istraživane populacije se mogu grupirati u skupine s najmanjim stupnjem razdvojenosti. Mantelovim testom komparacije utvrđene su statistički značajne korelacije između pojedinih molekularnih, morfoloških i fitokemijskih svojstava ploda, lista te ploda i lista istraživanih populacija. Obzirom na dobre morfološke i fitokemijske značajke ističu se plodovi populacije Vis, Šolta, Brač i Šipan. Plodovi populacije Orašac visokog su antioksidativnog potencijala i dobri izvori minerala.

Abstract (english)

Since there is a limited knowledge about croatian populations of a carob tree (Ceratonia siliqua L.), a nutritively and protectively high-quality plant, this research was conducted to determine the morphological, genetic and phytochemical traits of the carob populations in Croatia. The morphometric investigations of carob were made on the samples of pods and leaves, while the genetic variability was determined using AFLP analysis of genomic DNA isolated from the leaf. The content of total phenols, flavonoids and tannins, as well as antioxidant potential of the pods and the leaves, was determined spectrophotometrically. Content of macro and microelements was determined by using ICP-AES techniques. The research revealed a significant variability of the morphological and phytochemical traits of pods and leaves of the investigated carob populations. A molecular AFLP analysis showed considerable variability in the studied populations, because the variance between individuals within a population is much higher (75.51%) than the variance between populations. Despite a significant statistical variability, UPGMA cluster analysis of the studied populations, can be grouped in the lowest degree of separation. The Mantel test revealed significant correlations between molecular, morphological and phytochemical properties of the pods and leaves in the studied populations. Populations of Vis, Šolta, Brač and Šipan have good morphological and phytochemical features of the pods. The pods of the populations Vis and Orašac have the highest antioxidant potential and are a good source of the minerals.

• Keywords: rogač; populacija; morfologija; AFLP analiza; fitokemijske značajke
• Keywords (english): Carob tree; populations; morphology; AFLP analysis; phytochemical traits
• Language: croatian
• URN:NBN: urn:nbn:hr:217:965963
• Study programme: Title: Biology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2019-12-31
• Created on: 2017-07-06 12:01:32