Utjecaj trodimenzionalnih polimernih nanostruktura na razvoj primarnih živčanih stanica

master's thesis

• Title (english): Influence of three-dimensional polymeric nanostructures on the development of primary neurons
• Author: Frano Miloš
• Mentor: Dirk Mayer (mentor)
• Mentor: Maja Matulić (mentor)
• Committee member: Tomislav Ivanković (predsjednik povjerenstva)
• Committee member: Nataša Bauer (član povjerenstva)
• Committee member: Maja Matulić (član povjerenstva)
• Committee member: Željka Vidaković-Cifrek (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2017-05-30, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Površine sa specifičnom topografijom imaju potencijal u poboljšanju sučelja između stanica i biomedicinskih uređaja poput neuralnih implantata i biosenzora te bi mogle omogućiti liječenje različitih neuroloških poremećaja – sljepoće, paralize, Parkinsonove bolesti. Napredak nanotehnologije omogućio je izradu supstrata s točno definiranim mehaničkim i morfološkim svojstvima. Topografija izvanstaničnog okoliša utječe na staničnu morfologiju i razvoj te se može koristiti za kontroliranje stanične... More adhezije i diferencijacije. Cilj ovog rada je istražiti utjecaj površinske topografije na razvoj primarnih živčanih stanica. Primarne živčane stanice su izolirane iz korteksa mozga embrija štakora te kultivirane na supstratima polimera OrmoComp s nano- i mikrostupovima. Supstrati od polimera OrmoComp su podržavali rast živčanih stanica te nisu imali štetan utjecaj na njihovu vijabilnost. Nano- i mikrostupovi su poticali usmjereni rast živčanih stanica te njihovo poravnanje s topografijom pri čemu je ovaj efekt bio manje izražen kod stupova s većim promjerom i većim međusobnim razmakom. Pritom je povećanje visine stupova povećalo efekt usmjerenog rasta aksona te rezultiralo dužim i pravilnije raspoređenim aksonima. Osim toga, aksoni na topografijama su istraživali veći prostor tijekom rasta te su rasli brže i efikasnije što je rezultiralo povećanjem njihove duljine. Ovaj rad pokazuje da supstrati polimera OrmoComp s nano- i mikrostupovima podržavaju i kontroliraju rast kortikalnih živčanih stanica. Također, povećani i usmjereni rast aksona pokazuje potencijal ovih supstrata u regeneraciji oštećenog živčanog tkiva. Less

Abstract (english)

Surfaces with a defined topography could improve the interface between cells and biomedical devices such as neural implants and biosensors and enable treatment of various neurological disorders – blindness, paralysis, Parkinson’s disease. Advances in nanofabrication technologies have allowed generation of substrates with defined mechanical and morphological properties. Topography of the extracellular environment affects cell morphology and development and can be used to control adhesion and... More differentiation. The aim of this thesis was to investigate the influence of topography on the development of embryonic cortical neurons. Cortical neurons isolated from rat embryos were cultured on OrmoComp polymer substrates with nano- and micropillars. OrmoComp substrates supported neuronal growth without detrimental effects on cell viability. Nano- and microscale pillars enhanced neurite growth and alignment to the topography, an effect that diminished with increasing pillar diameter and spacing. Moreover, an increase in pillar height largely enhanced the axon guiding effect resulting in longer and more aligned axons. Live cell imaging revealed that axons growing on different topographies explored a larger area and had a more rapid and directional growth which resulted in an increase in axon length. This study demonstrates the suitability of OrmoComp nano- and micropillar substrates for supporting and controlling growth of cortical neurons. Furthermore, enhanced axon elongation and topographical guidance demonstrate the potential of these substrates as tissue engineering scaffolds for regeneration of damaged nerves. Less

• Keywords: topografija; primarne živčane stanice; nanostrukture; kontaktno usmjeravanje; neuralni implantati; regeneracija aksona; mikroskopija u stvarnom vremenu
• Keywords (english): topography; primary neuronal cells; nanostructures; contact guidance; neural implants; axon regeneration; real-time microscopy
• Language: croatian
• URN:NBN: urn:nbn:hr:217:965770
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2019-12-31
• Created on: 2017-09-07 08:08:14