Inducibilna i reverzibilna razgradnja proteinskog kompleksa za stvaranje mikroRNA u diferencirajućim ljudskim matičnim stanicama

master's thesis

• Title (english): Inducible and reversible disruption of the microRNA machinery in differentiating human stem cells
• Author: Marta Žužić
• Mentor: Volker Busskamp (mentor)
• Mentor: Petra Korać (mentor)
• Committee member: Petra Korać (predsjednik povjerenstva)
• Committee member: Željka Vidaković-Cifrek (član povjerenstva)
• Committee member: Tomislav Ivanković (član povjerenstva)
• Committee member: Maja Matulić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2016-12-13, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

mikroRNA (miRNA) su razred malih, nekodirajućih RNA koje reguliraju gensku eskpresiju na posttranskripcijskoj razini. Poznato je da reguliraju mnogobrojne biološke procese i stanične funkcije. Mnoge miRNA su i same dinamično regulirane tijekom razvoja centralnog živčanog sustava te različito raspoređene u odraslom mozgu, što ukazuje na njihovu esencijalnu ulogu u razvoju i funkciji živčanog sustava. miRNA profil se mijenja i tijekom in vitro diferencijacije ljudskih induciranih pluripotentnih matičnih stanica u bipolarne neurone, no njihova funkcija tijekom tog procesa i dalje nije u potpunosti jasna. Za istraživanje utjecaja miRNA tijekom neurogeneze i vremenskog aspekta njihove regulacije, koristeći tehnologiju CRISPR/Cas9, uspostavljen je degradirajući sustav baziran na auksinu za inducibilni prekid puta obrade miRNA u stanicama iNGN.

Abstract (english)

microRNAs (miRNAs) are a class of small, noncoding RNAs that regulate gene expression at the post-transcriptional level. They have been demonstrated to regulate a lot of biological pathways and cellular functions. Many miRNAs are dynamically regulated during central nervous system development and are spatially expressed in adult brain indicating their essential roles in neural development and function. Furthermore, miRNA profile changes during in vitro differentiation of human induced pluripotent stem cells to bipolar neurons. However, their function during the process is not yet fully known. To study the influence of miRNAs during neurogenesis and the temporal aspect of this regulation, the auxin-based degron system for inducible disruption of the miRNA processing machinery was established in iNGN cells, using CRISPR/Cas9 technology.

• Keywords: mikroRNA; degradirajući sustav baziran na auksinu; DGCR8; stanice iNGN
• Keywords (english): microRNA; auxin-based degron system; DGCR8; iNGN cells
• Language: croatian
• URN:NBN: urn:nbn:hr:217:257861
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-02-08 13:34:06